Recombinant Viral Dengue Virus 2 NS1 Protein, CF

Catalog # Availability Size / Price Qty
9439-DG-100
The Innovator Sereis.
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Citations (1)
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Recombinant Viral Dengue Virus 2 NS1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Human embryonic kidney cell, HEK293-derived viral Dengue Virus 2 NS1 protein
Asp776-Ala1127, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp776
Predicted Molecular Mass
41 kDa
SDS-PAGE
44-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9439-DG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity not tested The Innovator Sereis. View Larger

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dengue Virus 2 NS1

Dengue virus type2 non-structural protein 1 (DENV2 NS1)  is an approximately 50 kDa viral glycoprotein (1). Dengue viruses are mosquito-borne flaviviruses that exist in nature as four distinct serotypes (DENV1-4) (2). Dengue virus NS1 can be found associated with cell membranes, within cells and at the cell surface, or as secreted extracellular species. The secreted form of NS1 is known to be hexameric (1). Within cells, NS1 plays a significant role in virus replication and has been shown to co-localize with dsRNA and other components of viral replication complexes in viral-induced vesicle packets (1,3). Cell surface-associated and secreted NS1 are profoundly immunogenic, and both the protein itself and the antibodies it elicits have been shown to contribute to either protection or pathogenesis and innate immune evasion (1, 3). Mature DENV2 NS1 contains 352 amino acids (aa) and consists of 2 conserved N-glycosylation sites and 12 invariant cysteine residues that form six intramolecular disulfide bonds (3).Mature viral DENV2 NS1 shares 73%, 80%, and 69% aa sequence identity with DENV1, DENV3, and DENV4 NS1, respectively.

References
  1. Muller, D. et al. (2012) J. Gen. Virol. 93:771.
  2. VanBlargan, L.A. et al. (2013) PLOS Pathog. 9:e1003761.
  3. Flamand, M. et al. (1999) J. Virol. 73:6104.
Long Name
Dengue Virus 2 Thailand/16681/84 Polyprotein
Entrez Gene IDs
1494449 (Viral)
Alternate Names
DEN-2; Dengue NS1 protein; Dengue NS1; Dengue Virus 2 NS1; Dengue virus Serotype 2; Dengue virus Type 2; Dengue Virus; DENV2; DENV2-NS1

Citation for Recombinant Viral Dengue Virus 2 NS1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dengue NS1 antigen kit shows high sensitivity for detection of recombinant dengue virus-2 NS1 antigen spiked with Aedes aegypti mosquitoes
    Authors: PR Abraham, B R, PK N, A Kumar
    Scientific Reports, 2021;11(1):23699.
    Species: N/A
    Sample Types: Reference Standard

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