Simple Plex Cytokine Screening Panel

R&D Systems | Catalog # ST01E-PS-007487

Please use our newest generation panel: SPCKE-PS-010629.
R&D Systems
Discontinued Product
ST01E-PS-007487 has been discontinued. An alternative/replacement product is available: SPCKE-PS-010629. View all Multiplex Cytokine Assay Kits products.

Key Product Details

Features

The Simple Plex Human Multianalyte Assay is for the detection of human IL-1b, IL-12 p70, IL-10, IL-2, IFNg 3rd gen, TNF-a 2nd gen, IL-4 2nd gen, and IL-6 2nd gen in serum and plasma (EDTA/Heparin).

 
Sample Type & Volume Required Per Well Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity IL-1 beta 0.4 pg/mL; IL-12 p70 0.46 pg/mL; IL-10 0.58 pg/mL; IL-2 0.54 pg/mL; IFNg 3rd gen 0.17 pg/mL; TNF-a 0.3 pg/mL; IL-4 0.52 pg/mL; IL-6 0.28 pg/mL
Assay Range IL-1 beta 0.4 - 1,530 pg/mL; IL-12 p70 0.46 - 2,570 pg/mL; IL-10 0.58 - 2,212 pg/mL; IL-2 0.54 - 2,050 pg/mL; IFNg 3rd gen 0.17 - 4,000 pg/mL; TNF-a 0.3 - 1,160 pg/mL; IL-4 0.52 - 2,020 pg/mL; IL-6 0.28 - 2,652 pg/mL

Species

Human

Applications

Simple Plex Automated Immunoassay

Product Summary for Simple Plex Cytokine Screening Panel

Product Specifications

Reactivity Human
Specificity Natural and recombinant human IL-1b, IL-12 p70, IL-10, IL-2, IFNg 3rd gen, TNF-a 2nd gen, IL-4 2nd gen, and IL-6 2nd gen.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
IL-1 beta/IL-1F2 n 16 16 16 16
Mean (pg/mL) 8.66 446 8.42 410
Standard Deviation 0.427 16.5 0.342 23.3
CV (%) 4.9 3.7 4 5.7
IL-12 p70 n 16 16 16 16
Mean (pg/mL) 36.2 1657 33.2 1587
Standard Deviation 1.25 90 1.95 137
CV (%) 3.4 5.4 5.9 8.6
IL-10 n 16 16 16 16
Mean (pg/mL) 33 1591 33.2 1681
Standard Deviation 1.55 95.1 2.43 120
CV (%) 4.6 6 7.1 7.1
IL-2 n 16 16 16 16
Mean (pg/mL) 15 648 13 608
Standard Deviation 0.8 32.7 1.13 34
CV (%) 5.3 5.1 8.7 5.6
IFN-g n 16 16 16 16
Mean (pg/mL) 21.1 1175 23.4 1192
Standard Deviation 1.48 33.4 2.52 99.4
CV (%) 7 2.8 10.8 8.3
TNF-a n 16 16 16 16
Mean (pg/mL) 6.99 383 0.662 36.9
Standard Deviation 0.345 20.6 7.81 408
CV (%) 4.9 5.4 8.5 9
IL-4 n 16 16 16 16
Mean (pg/mL) 15.9 621 13.5 631
Standard Deviation 1.2 60.8 1.55 77.1
CV (%) 7.5 9.8 11.5 12.2
IL-6 n 16 16 16 16
Mean (pg/mL) 37.9 1763 38.7 1709
Standard Deviation 0.804 108 3.95 163
CV (%) 2.1 6.1 10.2 9.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

  Sample Type Average % Recovery Range %
IL-1 beta EDTA Plasma (n=4) 106 97-120
Heparin Plasma (n=4) 99 99-112
Serum (n=4) 105 96-112
IL-12 p70 EDTA Plasma (n=6) 87 73-117
Serum (n=6) 81 73-90
IL-10 EDTA Plasma (n=6) 105 88-113
Heparin Plasma (n=6) 102 89-118
Serum (n=6) 94 88-98
IL-2 EDTA Plasma (n=4) 86 69-100
Heparin Plasma (n=4) 90 70-103
Serum (n=4) 92 69-102
IFN-gamma EDTA Plasma (n=4) 89 88-90
Heparin Plasma (n=4) 90 83-99
Cerebrospinal Fluid (n=4) 94 91-98
Serum (n=4) 95 83-102
TNF-alpha EDTA Plasma (n=4) 97 89-105
Heparin Plasma (n=4) 89 77-96
Cerebrospinal Fluid (n=6) 109 103-115
Serum (n=4) 99 92-108
IL-4 EDTA Plasma (n=4) 88 83-92
Heparin Plasma (n=4) 87 84-89
Serum (n=4) 96 94-99
IL-6 EDTA Plasma (n=6) 95 92-98
Heparin Plasma (n=6) 93 91-94
Serum (n=6) 94 86-98
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Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.

Additional Multiplex Cytokine Assay Kits Products

Product Documents for Simple Plex Cytokine Screening Panel

Certificate of Analysis

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Product Specific Notices for Simple Plex Cytokine Screening Panel

For research use only

Citations for Simple Plex Cytokine Screening Panel

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