Simple Plex Cytokine Screening Panel

R&D Systems | Catalog # SPCKE-PS-010629

Simple Plex 32x8 panel containing IL-1b, IL-12 p70, IL-10 2nd Gen, IL-4 2nd Gen, IL-2, IFNg 3rd Gen, TNF-a 2nd Gen, and IL-6 3rd Gen with sample diluent SD13. Part # starting with ST01 ships in 3-5 days. Sample type indicated with PS (Plasma/Serum).
R&D Systems
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Key Product Details

Features

The Simple Plex Human Multianalyte Assay is for the detection of human IL-1b, IL-12 p70, IL-10 2nd gen, IL-2, IFNg 3rd gen, TNF-a 2nd gen, IL-4 2nd gen, and IL-6 3rd gen in serum and plasma (EDTA/Heparin).

 
Sample Type & Volume Required Per Well Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity IL-1 beta 0.4 pg/mL; IL-12 p70 0.46 pg/mL; IL-10 2nd gen 0.17pg/mL; IL-2 0.54 pg/mL; IFNg 3rd gen 0.17 pg/mL; TNF-a 0.3 pg/mL; IL-4 0.52 pg/mL; IL-6 3rd gen 0.11 pg/mL
Assay Range IL-1 beta 0.4 - 1,530 pg/mL; IL-12 p70 0.46 - 2,570 pg/mL; IL-10 2nd gen 0.38 - 1,446 pg/mL; IL-2 0.54 - 2,050 pg/mL; IFNg 3rd gen 0.17 - 4,000 pg/mL; TNF-a 0.3 - 1,160 pg/mL; IL-4 0.52 - 2,020 pg/mL; IL-6 3rd gen 0.28 - 2,652 pg/mL

Species

Human
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Kit Contents for Simple Plex Cytokine Screening Panel

Product Specifications

Reactivity Human
Specificity Natural and recombinant human IL-1b, IL-12 p70, IL-10 2nd gen, IL-2, IFNg 3rd gen, TNF-a 2nd gen, IL-4 2nd gen, and IL-6 3rd gen.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
IL-1b/IL-1F2 n 16 16 16 16
Mean (pg/mL) 8.66 446 8.42 410
Standard Deviation 0.427 16.5 0.342 23.3
CV (%) 4.9 3.7 4 5.7
IL-12 p70 n 16 16 16 16
Mean (pg/mL) 36.2 1657 33.2 1587
Standard Deviation 1.25 90 1.95 137
CV (%) 3.4 5.4 5.9 8.6
IL-10 2nd gen n 16 16 35 37
Mean (pg/mL) 8.89 462 10.8 477
Standard Deviation 0.193 16.0 0.940 24.5
CV (%) 2.2 3.5 8.7 5.1
IL-2 n 16 16 16 16
Mean (pg/mL) 15 648 13 608
Standard Deviation 0.8 32.7 1.13 34
CV (%) 5.3 5.1 8.7 5.6
IFN-g 3rd gen n 16 16 16 16
Mean (pg/mL) 21.1 1175 23.4 1192
Standard Deviation 1.48 33.4 2.52 99.4
CV (%) 7 2.8 10.8 8.3
TNF-a 2nd gen n 16 16 16 16
Mean (pg/mL) 6.99 383 0.662 36.9
Standard Deviation 0.345 20.6 7.81 408
CV (%) 4.9 5.4 8.5 9
IL-4 2nd gen n 16 16 16 16
Mean (pg/mL) 15.9 621 13.5 631
Standard Deviation 1.2 60.8 1.55 77.1
CV (%) 7.5 9.8 11.5 12.2
IL-6 3rd gen n 16 16 40 40
Mean (pg/mL) 23.1 876 23.6 1008
Standard Deviation 0.899 72.1 1.82 77.7
CV (%) 3.9 8.2 7.7 7.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

  Sample Type Average % Recovery Range %
IL-1b EDTA Plasma (n=4) 106 97-120
Heparin Plasma (n=4) 99 99-112
Serum (n=4) 105 96-112
IL-12 p70 EDTA Plasma (n=6) 87 73-117
Serum (n=6) 81 73-90
IL-10 2nd gen EDTA Plasma (n=4) 91 84-93
Heparin Plasma (n=4) 91 79-95
CCM (n=2) 101 100-103
Serum (n=4) 95 93-97
IL-2 EDTA Plasma (n=4) 86 69-100
Heparin Plasma (n=4) 90 70-103
Serum (n=4) 92 69-102
IFN-g 3rd gen EDTA Plasma (n=4) 89 88-90
Heparin Plasma (n=4) 90 83-99
Cerebrospinal Fluid (n=4) 94 91-98
Serum (n=4) 95 83-102
TNF-a 2nd gen EDTA Plasma (n=4) 97 89-105
Heparin Plasma (n=4) 89 77-96
Cerebrospinal Fluid (n=6) 109 103-115
Serum (n=4) 99 92-108
IL-4 2nd gen EDTA Plasma (n=4) 88 83-92
Heparin Plasma (n=4) 87 84-89
Serum (n=4) 96 94-99
IL-6 3rd gen EDTA Plasma (n=4) 92 86-102
Heparin Plasma (n=4) 87 85-90
Serum (n=4) 94 90-99
CCM (n=2) 104 103-106
Saliva (n=4) 102 102-102
Urine (n=4) 98 93-104

Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.

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Product Documents for Simple Plex Cytokine Screening Panel

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Certificate of Analysis

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Product Specific Notices for Simple Plex Cytokine Screening Panel

For research use only

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