Simple Plex Discovery Human MBL Assay

R&D Systems | Catalog # SPCKB-PS-012016

Simple Plex Human MBL assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

Customize My Cartridge on Bio-Techne

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com

Customize My Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

1.51 pg/mL

Assay Range

3.7-14130 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human MBL Assay Summary

Simple Plex Discovery assay is for the detection of human Mannan-Binding Lectin (MBL) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all MBL Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human MBL.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 23 24
Mean (pg/mL) 87.3 4484 89.4 4324
Standard Deviation 1.60 159 4.37 206
CV% 2.6 3.5 4.9 4.8

Recovery for Simple Plex Discovery Human MBL Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 90 88-93

Linearity

Samples containing and/or spiked with high concentrations of recombinant human MBL were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human MBL Ella

Scientific Data Images for Simple Plex Discovery Human MBL Assay

Simple Plex Discovery Human MBL Ella Assay

Simple Plex Discovery Human MBL Ella Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MBL

Mannose/mannan-binding lectin (MBL) is a secreted glycoprotein in the collectin family of pattern-recognition molecules. It contains a cysteine-rich region, a collagen-like segment, and a C-type lectin domain that binds to neutral bacterial carbohydrates. The collagen-like region mediates MBL association into disulfide-stabilized trimers which further associate into complexes containing three or four copies of the basic trimer. MBL is secreted by hepatocytes and opsonizes bacteria through interactions with microbial carbohydrates. MBL multimers can associate with the serine proteases MASP-1, -2, and -3 and promote their cleavage of Complement Component C3. Proteolytic cleavage of C3 triggers activation of the complement system and formation of the membrane attack complex, leading to destruction of opsonized bacteria. Opsonized apoptotic material is cleared by the scavenger receptor CD91.

Long Name

Mannose Binding Lectin

Alternate Names

COLEC1, Collectin-1, MBL2, MBL2D, MBP1

Entrez Gene IDs

4153 (Human)

Gene Symbol

MBL2

Additional MBL Products

Product Documents for Simple Plex Discovery Human MBL Assay

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human MBL Assay

For research use only

Citations for Simple Plex Discovery Human MBL Assay

View all citations for the Simple Plex platform on Bio-Techne.com.

Please visit our Instrument Citation Database on Bio-Techne.com to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Discovery Human MBL Assay

There are currently no reviews for this product. Be the first to review Simple Plex Discovery Human MBL Assay and earn rewards!

Have you used Simple Plex Discovery Human MBL Assay?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card