Simple Plex Discovery Human Serpin F1/PEDF Assay

R&D Systems | Catalog # SPCKB-PS-015287

Simple Plex Discovery Human Serpin F1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

Customize My Cartridge on Bio-Techne

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com

Customize My Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (0 µL)

Sensitivity

4.21 pg/mL

Assay Range

7.86-12000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Serpin F1/PEDF Assay Summary

Simple Plex Discovery assay is for the detection of human Serpin F1/PEDF in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Serpin F1/PEDF Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Serpin F1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Serpin F1/PEDF assay.

Inter-Assay Precision (Precision between assays) Replicates of each Serpin F1/PEDF control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Serpin F1/PEDF.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 85.4 4354 97.8 4477
Standard Deviation 2.96 157 7.23 190
CV% 3.5 3.6 7.4 4.3

Recovery for Simple Plex Discovery Human Serpin F1/PEDF Assay

Recovery for the human Serpin F1/PEDF assays was evaluated at three different spiked concentrations of Serpin F1/PEDF protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 112 111-113

Linearity

Samples containing and/or spiked with high concentrations of Serpin F1/PEDF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human Serpin F1/PEDF Assay Linearity

Scientific Data Images for Simple Plex Discovery Human Serpin F1/PEDF Assay

Simple Plex Human Serpin F1/PEDF Assay Standard Curve

Simple Plex Human Serpin F1/PEDF Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Serpin F1/PEDF Assay. Assay range is 7.86-12,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin F1/PEDF

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Long Name

Serpine F1/Pigment Epithelium-derived Factor

Alternate Names

EPC-1, PEDF

Entrez Gene IDs

5176 (Human); 20317 (Mouse); 287526 (Rat)

Gene Symbol

SERPINF1

Additional Serpin F1/PEDF Products

Product Documents for Simple Plex Discovery Human Serpin F1/PEDF Assay

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human Serpin F1/PEDF Assay

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Simple Plex Discovery Human Serpin F1/PEDF Assay

View all citations for the Simple Plex platform on Bio-Techne.com.

Please visit our Instrument Citation Database on Bio-Techne.com to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Discovery Human Serpin F1/PEDF Assay

There are currently no reviews for this product. Be the first to review Simple Plex Discovery Human Serpin F1/PEDF Assay and earn rewards!

Have you used Simple Plex Discovery Human Serpin F1/PEDF Assay?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card