Simple Plex Human Angiopoietin-1 Cartridge, 2nd Gen

R&D Systems | Catalog # SPCKB-PS-003640

Simple Plex Human Angiopoietin-1/ANG-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (5 µL), Platelet-poor EDTA Plasma (5 µL), Platelet-poor Heparin Plasma (5 µL), Saliva (5 µL)

Sensitivity

1.5 pg/mL

Assay Range

6.18-23560 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva)

Simple Plex Human Angiopoietin-1 Cartridge, 2nd Gen Summary

Simple Plex assay is for the detection of human Angiopoietin-1 in cell culture supernatant (CCS), serum, platelet-poor plasma (EDTA/Heparin), and saliva in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Angiopoietin-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Angiopoietin-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Angiopoietin-1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Angiopoietin-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Angiopoietin-1.

Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 70 70
Mean (pg/mL) 345 15920 378 17677
Standard Deviation 16.6 778 53.9 2020
CV% 4.8 4.9 14.2 11.4

Recovery for Simple Plex Human Angiopoietin-1 Cartridge, 2nd Gen

Recovery was not necessary as human Angiopoietin-1 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of Angiopoietin-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human Angiopoietin-1 Assay Linearity

Scientific Data Images for Simple Plex Human Angiopoietin-1 Cartridge, 2nd Gen

Simple Plex Human Angiopoietin-1 Assay Standard Curve

Simple Plex Human Angiopoietin-1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Angiopoietin-1 Assay. Assay range is 6.18-23,560 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system. It signals through the receptor tyrosine kinase Tie-2 and binds a variety of integrins and the extracellular matrix. Angiopoietin-1 induces the development and branching of blood vessels, promotes vascular endothelial cell motility during angiogenesis, and enhances vascular integrity. It can suppress several VEGF-induced effects on the vasculature. Angiopoietin-1 can protect against pulmonary arterial hypertension, reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium, and enhance tumor progression and metastasis.

Alternate Names

ANGPT1

Entrez Gene IDs

284 (Human); 11600 (Mouse)

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Simple Plex Human Angiopoietin-1 Cartridge, 2nd Gen

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Angiopoietin-1 Cartridge, 2nd Gen

For research use only

Citations for Simple Plex Human Angiopoietin-1 Cartridge, 2nd Gen

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