Simple Plex Human Cyr61/CCN1 Cartridge

R&D Systems | Catalog # SPCKB-PS-002307

Simple Plex Human Cyr61/CCN1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 µL), EDTA Plasma (5 µL), Heparin Plasma (5 µL)

Sensitivity

1.61 pg/mL

Assay Range

4.86-18532 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Cyr61/CCN1 Cartridge Summary

Simple Plex assay is for the detection of human Cysteine-rich angiogenic inducer 61 (Cyr61) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Cyr61/CCN1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Cyr61.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Cyr61 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Cyr61 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Cyr61.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 46 43
Mean (pg/mL) 158 6549 159 6772
Standard Deviation 9.33 499 15.8 618
CV% 5.9 7.6 9.9 9.1

Recovery for Simple Plex Human Cyr61/CCN1 Cartridge

Recovery for the human Cyr61 assays was evaluated at three different spiked concentrations of Cyr61 protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 100 91-111
Heparin Plasma (n=4) 96 83-103
Serum (n=4) 95 85-105

Linearity

Samples containing and/or spiked with high concentrations of human Cyr61 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Cyr61 Assay Linearity

Scientific Data Images for Simple Plex Human Cyr61/CCN1 Cartridge

Simple Plex Human Cyr61 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Cyr61 Assay. Assay range is 4.86-18,532 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cyr61/CCN1

Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa matricellular glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis through interactions with integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Alternate Names

CCN1, GIG1, IGFBP-10

Entrez Gene IDs

3491 (Human); 16007 (Mouse); 83476 (Rat)

Gene Symbol

CCN1

Additional Cyr61/CCN1 Products

Product Documents for Simple Plex Human Cyr61/CCN1 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Cyr61/CCN1 Cartridge

For research use only

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