Simple Plex Human Vitamin D BP Cartridge

R&D Systems | Catalog # SPCKB-PS-000541

Simple Plex Human Vitamin D BP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (25 µL)

Sensitivity

47.6 pg/mL

Assay Range

232-56710 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human Vitamin D BP Cartridge Summary

Simple Plex assay is for the detection of human Vitamin D Binding Protein (Vitamin D BP) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Vitamin D BP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Vitamin D BP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with other substances.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Vitamin D BP assay.

Inter-Assay Precision (Precision between assays) Replicates of each Vitamin D BP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Vitamin D BP.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 51 54
Mean (pg/mL) 293 14947 311 18273
Standard Deviation 20.3 820 29.5 952
CV% 6.9 5.5 9.5 5.2

Recovery for Simple Plex Human Vitamin D BP Cartridge

Recovery was not necessary as human Vitamin D BP shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Vitamin D BP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human Vitamin D BP Assay Linearity

Scientific Data Images for Simple Plex Human Vitamin D BP Cartridge

Simple Plex Human Vitamin D BP Assay Standard Curve

Simple Plex Human Vitamin D BP Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Vitamin D BP Assay. Assay range is 232-56,710 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Vitamin D BP

Vitamin D binding protein (VitD BP), also known as DBP and Gc-globulin, is a 58 kDa glycoprotein that circulates at high concentration in the serum and serves as a carrier protein for vitamin D. The transport of vitamin D by VitD BP is important for the function of a wide variety of tissues. VitD BP binds both the 25(OH) and the hormonally active 1,25(OH)2 forms of vitamin D. VitD BP is primarily expressed in hepatocytes and to a lesser extent in the kidney. It delivers vitamin D into cells by Megalin-mediated endocytosis. A selectively deglycosylated form of VitD BP known as macrophage activating factor (MAF) blocks the angiogenic effects of FGF basic, VEGF, and Angiopoietin 2. VitD BP enhances the chemotaxis of monocytes and neutrophils to the activated complement component C5a or C5a des Arg (a C-terminally processed form of C5a). The chemotactic cofactor property of VitD BP is eliminated by binding to 1,25(OH)2 vitamin D, but it is not altered by binding to 25(OH) vitamin D or actin.

Long Name

Vitamin D Binding Protein

Alternate Names

DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP

Entrez Gene IDs

2638 (Human); 14473 (Mouse); 24384 (Rat)

Gene Symbol

GC

Additional Vitamin D BP Products

Product Documents for Simple Plex Human Vitamin D BP Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Vitamin D BP Cartridge

For research use only

Citations for Simple Plex Human Vitamin D BP Cartridge

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