Recombinant Single-stranded DNA Binding Protein

Novus Biologicals | Catalog # NBP2-35314

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

Q97W73

Applications

SDS-PAGE
View all Single-stranded DNA Binding Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 148 amino acids corresponding to Single-stranded DNA Binding ProteinSource: E. coli

Uniprot ID: Q97W73

Amino Acid Sequence: MEEKVGNLKP NMESVNVTVR VLEASEARQI QTKNGVRTIS EAIVGDETGR VKLTLWGKHA GSIKEGQVVK IENAWTTAFK GQVQLNAGSK TKIAEASEDG FPESSQIPEN TPTAPQQMRG GGRGFRGGGR RYGRRGGRRQ ENEEGEEE

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Single-stranded DNA Binding as determined by LAL method.

Predicted Molecular Mass

16.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Single-stranded DNA Binding Protein

SDS-PAGE: Recombinant Single-stranded DNA Binding Protein [NBP2-35314]

SDS-PAGE: Recombinant Single-stranded DNA Binding Protein [NBP2-35314]

SDS-Page: Recombinant Human, Other Single-stranded DNA Binding Protein [NBP2-35314] - Single-stranded DNA Binding Protein [NBP2-35314]

Formulation, Preparation, and Storage

NBP2-35314
Formulation 0.2 um filtered concentrated solution in 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM EDTA, 0.5 mM DTT.
Preservative 50% glycerol
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Format Carrier-Free
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: Single-stranded DNA Binding

Single-stranded DNA-binding protein, or SSB, binds to single-stranded regions of DNA to prevent premature annealing, to protect the single-stranded DNA from being digested by nucleases, and to remove secondary structure from the DNA to allow other enzymes to function effectively upon it. In molecular biology, SSB protein domain in bacteria are important in its function of maintaining DNA metabolism, more specifically DNA replication, repair and recombination. SSB proteins have been identified in organisms from viruses to humans. The only organisms known to lack them are thermoproteales, a group of extremophile archaea, where they have been displaced by the protein ThermoDBP. While many phage and viral SSBs function as monomers and eukaryotes encode heterotrimeric RPA (Replication Protein A), the best characterized SSB is that from the bacteria E. coli which, like most bacterial SSBs exists as a tetramer.

UniProt

Q97W73 (Other)

Additional Single-stranded DNA Binding Products

Product Documents for Recombinant Single-stranded DNA Binding Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Single-stranded DNA Binding Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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