Recombinant Human SOD1/Cu-Zn SOD Protein

Name: Recombinant Human SOD1/Cu-Zn SOD Protein
Brand: Novus Biologicals
SKU: NBC1-18544
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBC1-18544

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

View all SOD1/Cu-Zn SOD Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-154 of Human SOD1/Cu-Zn SOD

Source: E.coli

Amino Acid Sequence: MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

15.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 500 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SOD1/Cu-Zn SOD Protein

SDS-PAGE: Recombinant Human SOD1/Cu-Zn SOD Protein [NBC1-18544]

SDS-Page: Recombinant Human SOD1/Cu-Zn SOD Protein [NBC1-18544] - SOD1, 15.9 kDa (154 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation, and Storage

NBC1-18544

Formulation	20 mM Tris-HCl buffer (pH 7.5), 10% Glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOD1/Cu-Zn SOD

Superoxide dismutase 1(SOD1) binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. The encoded protein neutralizes supercharged oxygen molecules, which can damage cells if their levels are not controlled. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS). Recombinant SOD1 was expressed in E.coli and purified by conventional chromatography techniques.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

Additional SOD1/Cu-Zn SOD Products

Product Documents for Recombinant Human SOD1/Cu-Zn SOD Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human SOD1/Cu-Zn SOD Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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