Sulfo-NHS-LC-Biotin

Name: Sulfo-NHS-LC-Biotin
Brand: Tocris Bioscience
SKU: 7302
Price: 213 USD
Availability: InStock

Tocris Bioscience | Catalog # 7302

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Citations (3)

Product Documents

Amine reactive biotinylation reagent, cell impermeable

Product Description
Product Specifications
Solubility
Preparing Stock Solutions
Calculators
Background References
Product Documents
Specific Notices

Key Product Details

Description

Amine reactive biotinylation reagent, cell impermeable

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Product Description

Sulfo-NHS-LC-Biotin is a water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. Commonly used to label proteins and antibodies. Does not penetrate membrane. Reactive in pH 7.0-9.0, solubility 10 mg/mL in water and DMF.

Product Specifications for Sulfo-NHS-LC-Biotin

Molecular Weight

556.58

Formula

C₂₀H₂₉N₄NaO₉S₂

Storage

Store at -20°C

Chemical Name

Sodium 2,5-dioxo-1-((6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)oxy)pyrrolidine-3-sulfonate

CAS Number

191671-46-2

PubChem ID

71571497

InChI Key

JJGWLCLUQNFDIS-GTSONSFRSA-M

SMILES

[H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCC(ON3C(CC(S([O-])(=O)=O)C3=O)=O)=O)=O.[Na+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	55.66	100
	DMSO	55.66	100

Preparing Stock Solutions for Sulfo-NHS-LC-Biotin

The following data is based on the product molecular weight 556.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.80 mL	8.98 mL	17.97 mL
5 mM	0.36 mL	1.80 mL	3.59 mL
10 mM	0.18 mL	0.90 mL	1.80 mL
50 mM	0.04 mL	0.18 mL	0.36 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 3 publications citing the usage of this product.

Xu and Wegner Multifunctional streptavidin-biotin conjugates with precise stoichiometries. Chem.Sci.
Karunakaran Butyrophilin-2A1 directly binds germline-encoded regions of the Vγ9Vδ2 TCR and is essential for phosphoantigen sensing. Immunity 2020 PMID: 32155411
Takayama Long-term drug modification to the surface of mesenchymal stem cells by the avidin-biotin complex method. Sci.Rep 2017 PMID: 29208980

Product Documents for Sulfo-NHS-LC-Biotin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Sulfo-NHS-LC-Biotin

For research use only

Citations for Sulfo-NHS-LC-Biotin

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