Catalog Number: 5118
Alternate Names: FM4-64
Chemical Name: 4-[6-[4-(Diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]pyridinium dibromide
Biological Activity
Fluorescent dye; stains synaptic vesicles. Becomes fluorescent when incorporated into plasma membrane, used to follow synaptic activity at the synapse or neuromuscular junction. Excitation and emission spectra are ~ 515 and 640 nm respectively.
Technical Data
  • M.Wt:
    607.51
  • Formula:
    C30H45Br2N3
  • Solubility:
    Soluble to 10 mM in DMSO
  • Purity:
    >95%
  • Storage:
    Store at -20°C
  • CAS No:
    162112-35-8
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Additional Information
Licensing Caveats:
SynaptoRed is a trademark of Biotium, Inc.
Background References
  1. Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening.
    Daniel et al.
    Nat.Protoc., 2012;7:1439
  2. Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae.
    Fischer-Parton et al.
    J.Microsc., 2000;198:246
  3. Vesicular sterols are essential for synaptic vesicle cycling.
    Dason et al.
    J.Neurosci., 2010;30:15856

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