A homodimeric protein consisting of two 59 amino acid chains corresponding to TFF3, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds Source: E. coli

Uniprot ID: Q07654

Amino Acid Sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of TFF3 as determined by LAL method.

Predicted Molecular Mass

13.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Trefoil Factor 3 protein is fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ug/ml, corresponding to a specific activity of > 100 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TFF3 Protein

SDS-PAGE: Recombinant Human TFF3 Protein [NBP2-35060]

SDS-Page: Human TFF3 Protein [NBP2-35060]

Formulation, Preparation, and Storage

NBP2-35060

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TFF3

Trefoil Factor 3 (TFF3), also known as ITF and P1.B, plays an important role in epithelial regeneration and wound healing. It is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach. It is upregulated in response to a range of gastrointestinal epithelial disruptions and induces the migration of biliary, bronchial, and intestinal epithelial cells. Its upregulation is also associated with and enhances tumor cell invasion and metastasis. TFF3 promotes angiogenesis and enhances VEGF upregulation in tumor cells. It can form disulfide-linked dimers or associate into disulfide-linked complexes with other intestinal mucous proteins including FCGBP and MUC-2.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Gene Symbol

TFF3

UniProt

Q07654

Additional TFF3 Products

Product Documents for Recombinant Human TFF3 Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human TFF3 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Inflammatory Mediators

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Recombinant Human TFF3 Protein

Novus Biologicals | Catalog # NBP2-35060

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