Thapsigargin

Tocris Bioscience | Catalog # 1138

Potent inhibitor of SERCA ATPase
Tocris Bioscience

Key Product Details

Description

Potent inhibitor of SERCA ATPase
View all Ca2+-ATPase Inhibitors »

Product Description

Thapsigargin is a potent inhibitor of sarco/endoplasmic reticulum Ca2+-ATPases (SERCA) that causes endoplasmic reticulum (ER) stress. Thapsigargin can be used to induce autophagy in mammalian cells. In vitro, thapsigargin blocks infection of immortilized and primary human cells with respiratory syncytial virus (RSV), SARS-CoV-2 and influenza A. Thapsigargin protects mice against lethal influenza A viral infection and reduces virus titres in the lungs of treated mice.

Product Specifications for Thapsigargin

Molecular Weight

650.76

Formula

C34H50O12

Storage

Store at -20°C

Purity

≥97% (HPLC)

Chemical Name

(3S,3aR,4S,6S,6AR,7S,8S,9bS)-6-(Acetyloxy)-2,3,3a,4,5,6,6a,7,8,9b-decahydro-3,3a-dihydroxy-3,6,9-trimethyl-8-[[(2Z)-2-methyl-1-oxo-2-butenyl]oxy]-2-oxo-4-(1-oxobutoxy)azuleno[4,5-b]furan-7-yl octanoate

CAS Number

67526-95-8

PubChem ID

446378

InChI Key

IXFPJGBNCFXKPI-FSIHEZPISA-N

SMILES

O=C(O[C@@H]2[C@@]3(O)[C@@H](OC([C@](C)3O)=O)C1=C(C)[C@H](OC(/C(C)=C\C)=O)[C@@H](OC(CCCCCCC)=O)[C@@]([H])1[C@](OC(C)=O)(C)C2)CCC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 65.08 100

Preparing Stock Solutions for Thapsigargin

The following data is based on the product molecular weight 650.76.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.54 mL 7.68 mL 15.37 mL
5 mM 0.31 mL 1.54 mL 3.07 mL
10 mM 0.15 mL 0.77 mL 1.54 mL
50 mM 0.03 mL 0.15 mL 0.31 mL

Calculators

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Product Documents for Thapsigargin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Thapsigargin

For research use only

Related Research Areas

Ion Channels and Regulators

Customer Reviews for Thapsigargin (7)

4.4 out of 5
7 Customer Ratings
5 Stars
57%
4 Stars
29%
3 Stars
14%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 7 reviews Showing All
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  • Good activator for SOCE
    Name: Yuyang Sun
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Macrophage
    Verified Customer | Posted 05/30/2022
    This product significantly induced calcium release and store operated calcium entry.
    Thapsigargin 1138
  • Usage Information
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: KMS11 or OPM2
    Verified Customer | Posted 06/20/2020
    Dissolved in DMSO. We treated cells for 16 hr.
    100 nM
    Thapsigargin 1138
  • Thapsigargin Blocks Re-Uptake of Ca2+ Into ER leading to increased T-cell activation by IFNg
    Name: Li Zhu
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Primary Cd8+ T-cell
    Verified Customer | Posted 01/23/2019
    Thapsigargin was used to block SERCA in activated CD8+ T-cells. This maintained high intracellular Ca2+, leading to increased activation of CD8+ T-cells as measured by IFNg production via intracellular flow cytometry.
    Thapsigargin 1138
  • Intracellular calcium depletion
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Verified Customer | Posted 11/19/2018
    This compound is often used to deplete intracellular calcium stores in cell culture or other applications. It worked as expected in our tissue preparations.
  • Thapsigargin elicits store-operated Ca2+ entry after ER store depletion in RBL-2H3 cells.
    Name: Niroj Shrestha
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: RBL-2H3
    Verified Customer | Posted 10/27/2018
    Cytosolic Ca2+ sensitive indicator, Fura-2 was loaded in RBL-2H3 cells and excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm. ER store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry via STIM1/Orai1 pathway into the cells upon addition of 2 mM extracellular Ca2+. RBL-2H3 cells were loaded with FURA-2AM (2 μM) and Pluronic F127 (0.02%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.
    Thapsigargin 1138
  • XBP1 splicing
    Name: Preethi Vijayaraj
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: mesenchymal cells
    Verified Customer | Posted 05/01/2018
    Used it as a positive control to test if the mechanism of a novel small molecule (compound x) was to reduce ER stress. Cells were treated with 5um Thapsigargin for 24 hours, and splicing of XBP1 was determined by semi-quantitative RT-PCR
    Thapsigargin 1138
  • Thapsigargin calcium assay
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK 293T
    Verified Customer | Posted 12/12/2017
    for calcium release from the ER

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