Ultra Rainbow Calibration ERF Particle Set (6 peaks)

Novus Biologicals | Catalog # NBP3-11817

Novus Biologicals
100% Guarantee

Key Product Details

Applications

Flow Cytometry, Microscopy

Product Summary for Ultra Rainbow Calibration ERF Particle Set (6 peaks)

Ultra Rainbow Quantitative Particle Kit

  • Contains 6 intensities of Ultra Rainbow Fluorescent Beads with NIST assigned ERF (Equivalent Number of Reference Fluorophores) values based on a published procedure using NIST SRM 1934 and a calibrated laserbased CCD fluorimeter.
  • Provides microsphere reference standards for fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC) and Pacific Blue (PB) fluorescent channels using four different NIST reference fluorophores; fluorescein, Nile Red, APC and Coumarin 30.
  • Used during flow cytometry standardardization and calibration measurements for the further advancement of biological and clinical applications.
    • Particle size: 3.5 -3.9 micron



    Size: 6 x 2 mL

    Scientific Data Images for Ultra Rainbow Calibration ERF Particle Set (6 peaks)

    Ultra Rainbow Calibration ERF Particle Set (6 peaks) [NBP3-11817]
    Ultra Rainbow Calibration ERF Particle Set (6 peaks) [NBP3-11817]

    Formulation, Preparation, and Storage

    Concentration

    Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    Storage

    Store at 4C in the dark. Do not freeze.

    Background: Ultra Rainbow Calibration ERF Particle Set (6 peaks)

    Rainbow Calibration Particles are used to ascertain the relative voltage range for each flow cytometer detector. This mixture of fluorescent particles serves as a reliable and reproducible means of evaluating the sensitivity and resolution of flow cytometers.

    The relative number of fluorophores can been determined for every emission peak of the rainbow calibration particles in FL1 (FITC, MEFL), FL2 (RPE, MEPE), FL3 (RPE-Cy5, MEPCY) and FL4 (APC, MEAP) channels in order to plot the calibration graph. The calibration graph is used to check the linearity (dynamic range) of the photomultiplier tube (PMT) in each channel. Additionally, the relative number of fluorophores can be cross calibrated with cells or particles stained with a known number of spectral matching fluorophores such as FITC, PE, RPE-Cy5 to estimate the number of fluorophores on stained cells.

    Additional Ultra Rainbow Calibration ERF Particle Set (6 peaks) Products

    Product Documents for Ultra Rainbow Calibration ERF Particle Set (6 peaks)

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Product Specific Notices for Ultra Rainbow Calibration ERF Particle Set (6 peaks)

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

    Customer Reviews for Ultra Rainbow Calibration ERF Particle Set (6 peaks)

    There are currently no reviews for this product. Be the first to review Ultra Rainbow Calibration ERF Particle Set (6 peaks) and earn rewards!

    Have you used Ultra Rainbow Calibration ERF Particle Set (6 peaks)?

    Submit a review and receive an Amazon gift card!

    $25/€18/£15/$25CAN/¥2500 Yen for a review with an image

    $10/€7/£6/$10CAN/¥1110 Yen for a review without an image

    Submit a review
    Amazon Gift Card

    FAQs

    No product specific FAQs exist for this product.

    View all FAQs for Supplemental Kits and Reagents