beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.
Human beta ‑Catenin Fluorescein‑conjugated Antibody
R&D Systems | Catalog # IC13292F
Discontinued Product
IC13292F has been discontinued.
View all beta-Catenin products.
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 196624
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Product Specifications
Immunogen
E. coli-derived recombinant human beta -Catenin
Ala2-Leu781
Accession # P35222
Ala2-Leu781
Accession # P35222
Specificity
Detects human, mouse, and rat beta -Catenin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human beta ‑Catenin Fluorescein‑conjugated Antibody
Detection of beta ‑Catenin in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human beta -Catenin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC13292F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Applications for Human beta ‑Catenin Fluorescein‑conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Reviewed Applications
Read 1 review rated 5 using IC13292F in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-Catenin
Alternate Names
bCatenin, CTNNB1
Gene Symbol
CTNNB1
UniProt
Additional beta-Catenin Products
Product Documents for Human beta ‑Catenin Fluorescein‑conjugated Antibody
Product Specific Notices for Human beta ‑Catenin Fluorescein‑conjugated Antibody
For research use only
Customer Reviews for Human beta ‑Catenin Fluorescein‑conjugated Antibody (1)
5 out of 5
1 Customer Rating
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Blood-Brain Barrier and Immune Cell Transmigration: ICAM-1/CD54 Signaling Pathways
Blood-Brain Barrier and Immune Cell Transmigration: Pathways Overview
Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways
Blood-Brain Barrier and Immune Cell Transmigration: VEGF Signaling Pathways
Blood-Brain Barrier Pathway: Anatomy
HIF Enhancer Pathways
Notch Signaling Pathways
Wnt Signaling Pathways: beta-Catenin-dependent Wnt Signaling
HIF Enhancer Pathways
Notch Signaling Pathways
Wnt Signaling Pathways: beta-Catenin-dependent Wnt Signaling