Flow Cytometry Protocol for Staining Membrane Associated Proteins

This protocol is intended as a guide only.

This video protocol provides step-by-step guide on how to perform flow cytometry using antibodies from R&D Systems and Novus Biologicals to stain membrane-associated proteins in suspended cells. This protocol has been developed and optimized by R&D Systems Flow Cytometry Laboratory.

  • 0:40 Introduction
  • 1:00 Before We Begin
  • 1:10 Sample Preparation
  • 2:15 Experimental Setup
  • 2:30 Cell Preparation
  • 2:50 FC Blocking
  • 3:25 Adding Conjugated Antibody
  • 3:50 Wash Step
  • 5:05 Resuspending Cells
  • 5:20 Conclusion
View full written flow cytometry protocol for membrane staining

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