Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Bovine, Transgenic Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MFG-E8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MFG-E8 (R&D Systems, Catalog # 2805-MF)
Ala23-Cys463
Accession # P21956

Specificity

Detects mouse MFG-E8 in direct ELISAs and Western blots. In these formats, less than 2% cross‑reactivity with recombinant human MFG-E8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse MFG‑E8 Antibody

Detection of Mouse MFG‑E8 antibody by Western Blot.

Detection of Mouse MFG‑E8 by Western Blot.

Western blot shows lysates of mouse mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MFG-E8 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse MFG-E8 antibody by Simple WesternTM.

Detection of Mouse MFG‑E8 by Simple WesternTM.

Simple Western lane view shows lysates of recombinant mouse (rm) MFG-E8, loaded at 50 ng/mL. A specific band was detected for MFG-E8 at approximately 87 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human MFG-E8 by Western Blot

Detection of Human MFG-E8 by Western Blot

MFGE8 and NO Are Produced and Secreted by Co-cultured BV2 Microglia Only When Co-cultured in Contact with pFTAA+Neurons(A and B) Representative blot (A) ofMFGE8 in BV2 cells lysates cultured alone or co-cultured in direct contact, or via atranswell, with DRG neurons from 5-month-old P301S-tau, 5-month-old C57, 5-month-old Alz17 mice, or 2-month-old P301S mice.Asterisk: either another isoform ofMFGE8 or a breakdown product. (B) Densitometry of MFGE8 expression normalized to beta -actin; significantly more MFGE8 is produced only in contact co-cultures containing pFTAA+ neurons. Mean± SD, n = 3 independent experiments (*p < 0.05), twoway ANOVA, Dunnett’s correction.(C and D) Elevated MFGE8 (C) or NO (D) in medium conditioned by BV2 cells co-cultured in contact with DRG neurons from5-month-old P301S mice compared with 5-month-old Alz17, 5-month- old C57, or 2-month-old P301S mice (MFGE8: ****p < 0.0001versus all; NO: ****p < 0.0001 versus 5-month-old C57 or 2-month-old P301S mice; ***p < 0.001 versus 5-month-oldAlz17 mice). N.D., none detected. Mean ± SD, n = 3 independent experiments, two-way ANOVA, Bonferroni corrected.(E and F) Elevated MFGE8 (E) or NO (F) in medium conditioned by primary microglia from C57 mice co-cultured in contactwith DRG neurons from 5-month-old P301S mice; microglia from MFGE8 KO mice are negative controls. Mean ± SD, n = 3independent microglial preparations, one-way ANOVA, **p < 0.01 (MFGE8), *p < 0.05 (NO).(G) Increased MFGE8 immunostaining intensity in frontal motor cortex of 5-month-old P301S mice compared with 5-month-oldC57 mice; MFGE8 KO mouse is negative control; 25 μm section at inter- aural 5.12 mm, bregma 1.32 mm; brown, DAB; blue,cresyl violet. Scale bar, 130 mm. Inset, 65 μm.(H and I) Elevated MFGE8 (H) in 5-month-old P301S-tau brains. Lysatesfrom cortex(Ctx), brain stem (BS), and cerebellum(Cb) of 5-month-old C57, 5-month-old P301S-tau, and 5-month-old Alz17 mice probed with anti-MFGE8; MFGE8 KO brain lysate isnegative control. rec, recombinant mouse MFGE8. (I) Densitometry of MFGE8 expression normalized to beta -actin. Significantlyhigher MFGE8 expression in P301S versus C57BU6 Ctx (**p < 0.01), BS (****p < 0.0001), Cb (**p < 0.01), andP301SversusALz17BS (****p < 0.0001). Mean ± SD, n = 3 independent preparations, two-way ANOVA, Bonferronicorrected.(J) Elevated MFGE8 expression in brain extracts from cortex (Ctx) of FTDP-17T patients with two differentMAPT mutations (P301L, +3) or sporadic Pick’s disease but not in extracts from patients with C9orf72hexanucleotide expansions and TDP-43 aggregates. No expression is found in the cerebellum, where tau pathology is absent in allcases. Human milk MFGE8 is a positive control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30134156), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human MFG-E8 by Western Blot

Detection of Human MFG-E8 by Western Blot

MFG-E8 deficiency impairs wound closure and wound angiogenesis.a The serum levels of MF-E8 in HCs (n = 30), diabetes (n = 33), and DFU patients (n = 25) were detected with ELISA. b The serum levels of MFG-E8 in normal or diabetic mice (n = 6) post-wounding for 0, 3, 7, 14 days were determined by ELISA. c Western blot analysis of MFG-E8 expression in skin tissues of normal or diabetic mice after wound for 3 days, GAPDH as a loading control. d The serum fed glucose levels of WT (n = 24) or Mfge8−/− mice (n = 28) when treated with vehicle or STZ for 28 days were measured. e Representative digital imaging of wounds from age-matched diabetic WT or Mfge8−/− mice postwounding up to day 14. f Wound closures kinetics of diabetic WT and Mfge8−/− mice (n = 6). g Representative images of H&E-stained skin tissues from WT or Mfge8−/− mice injected with vehicle or STZ after wound for 3 days. h The amount of CD31+ epithelial cells (ECs) and alpha -smooth muscle actin ( alpha -SMA+) pericytes in wound skins of WT or Mfge8−/− mice treated with vehicle or STZ at day 14 after wound. i Quantification of alpha -SMA+ vessels in five random microscopic fields in n = 6 mice per group was performed. For all experiments, data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32963812), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human MFG-E8 by Western Blot

Detection of Human MFG-E8 by Western Blot

MFGE8 and NO Are Produced and Secreted by Co-cultured BV2 Microglia Only When Co-cultured in Contact with pFTAA+Neurons(A and B) Representative blot (A) ofMFGE8 in BV2 cells lysates cultured alone or co-cultured in direct contact, or via atranswell, with DRG neurons from 5-month-old P301S-tau, 5-month-old C57, 5-month-old Alz17 mice, or 2-month-old P301S mice.Asterisk: either another isoform ofMFGE8 or a breakdown product. (B) Densitometry of MFGE8 expression normalized to beta -actin; significantly more MFGE8 is produced only in contact co-cultures containing pFTAA+ neurons. Mean± SD, n = 3 independent experiments (*p < 0.05), twoway ANOVA, Dunnett’s correction.(C and D) Elevated MFGE8 (C) or NO (D) in medium conditioned by BV2 cells co-cultured in contact with DRG neurons from5-month-old P301S mice compared with 5-month-old Alz17, 5-month- old C57, or 2-month-old P301S mice (MFGE8: ****p < 0.0001versus all; NO: ****p < 0.0001 versus 5-month-old C57 or 2-month-old P301S mice; ***p < 0.001 versus 5-month-oldAlz17 mice). N.D., none detected. Mean ± SD, n = 3 independent experiments, two-way ANOVA, Bonferroni corrected.(E and F) Elevated MFGE8 (E) or NO (F) in medium conditioned by primary microglia from C57 mice co-cultured in contactwith DRG neurons from 5-month-old P301S mice; microglia from MFGE8 KO mice are negative controls. Mean ± SD, n = 3independent microglial preparations, one-way ANOVA, **p < 0.01 (MFGE8), *p < 0.05 (NO).(G) Increased MFGE8 immunostaining intensity in frontal motor cortex of 5-month-old P301S mice compared with 5-month-oldC57 mice; MFGE8 KO mouse is negative control; 25 μm section at inter- aural 5.12 mm, bregma 1.32 mm; brown, DAB; blue,cresyl violet. Scale bar, 130 mm. Inset, 65 μm.(H and I) Elevated MFGE8 (H) in 5-month-old P301S-tau brains. Lysatesfrom cortex(Ctx), brain stem (BS), and cerebellum(Cb) of 5-month-old C57, 5-month-old P301S-tau, and 5-month-old Alz17 mice probed with anti-MFGE8; MFGE8 KO brain lysate isnegative control. rec, recombinant mouse MFGE8. (I) Densitometry of MFGE8 expression normalized to beta -actin. Significantlyhigher MFGE8 expression in P301S versus C57BU6 Ctx (**p < 0.01), BS (****p < 0.0001), Cb (**p < 0.01), andP301SversusALz17BS (****p < 0.0001). Mean ± SD, n = 3 independent preparations, two-way ANOVA, Bonferronicorrected.(J) Elevated MFGE8 expression in brain extracts from cortex (Ctx) of FTDP-17T patients with two differentMAPT mutations (P301L, +3) or sporadic Pick’s disease but not in extracts from patients with C9orf72hexanucleotide expansions and TDP-43 aggregates. No expression is found in the cerebellum, where tau pathology is absent in allcases. Human milk MFGE8 is a positive control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30134156), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human MFG-E8 by Western Blot

Detection of Human MFG-E8 by Western Blot

The activation of NLRP3 inflammasome was aggravated in MFG-E8-deficient mice.a The infiltrated active caspase-1+ and IL-1 beta + macrophages in dermis of wound skins from WT or Mfge8−/− mice (n = 6) treated with vehicle or STZ, post-wounding at day 3, were determined with immunofluorescence. b Quantification of the expression of active caspase-1, caspase-1, and MFG-E8 in wound skin tissue from WT or Mfge8−/− mice (n = 6) treated with vehicle or STZ at day 3 post-wounding, beta -actin as loading control. c Calculation of the ratio of active caspase-1/caspase-1 in wound skin tissues from each group mice. d Infiltration of death cells into wound skin tissues of WT or Mfge8−/− mice (n = 6) post-wounding at day 3 was identified by TUNEL. Representative images were shown, original magnification ×200. e The average TUNEL-positive cells were counted at more than five random microscopic fields. For all experiments, data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32963812), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human MFG-E8 by Western Blot

Detection of Human MFG-E8 by Western Blot

MFGE8 and NO Are Produced and Secreted by Co-cultured BV2 Microglia Only When Co-cultured in Contact with pFTAA+Neurons(A and B) Representative blot (A) ofMFGE8 in BV2 cells lysates cultured alone or co-cultured in direct contact, or via atranswell, with DRG neurons from 5-month-old P301S-tau, 5-month-old C57, 5-month-old Alz17 mice, or 2-month-old P301S mice.Asterisk: either another isoform ofMFGE8 or a breakdown product. (B) Densitometry of MFGE8 expression normalized to beta -actin; significantly more MFGE8 is produced only in contact co-cultures containing pFTAA+ neurons. Mean± SD, n = 3 independent experiments (*p < 0.05), twoway ANOVA, Dunnett’s correction.(C and D) Elevated MFGE8 (C) or NO (D) in medium conditioned by BV2 cells co-cultured in contact with DRG neurons from5-month-old P301S mice compared with 5-month-old Alz17, 5-month- old C57, or 2-month-old P301S mice (MFGE8: ****p < 0.0001versus all; NO: ****p < 0.0001 versus 5-month-old C57 or 2-month-old P301S mice; ***p < 0.001 versus 5-month-oldAlz17 mice). N.D., none detected. Mean ± SD, n = 3 independent experiments, two-way ANOVA, Bonferroni corrected.(E and F) Elevated MFGE8 (E) or NO (F) in medium conditioned by primary microglia from C57 mice co-cultured in contactwith DRG neurons from 5-month-old P301S mice; microglia from MFGE8 KO mice are negative controls. Mean ± SD, n = 3independent microglial preparations, one-way ANOVA, **p < 0.01 (MFGE8), *p < 0.05 (NO).(G) Increased MFGE8 immunostaining intensity in frontal motor cortex of 5-month-old P301S mice compared with 5-month-oldC57 mice; MFGE8 KO mouse is negative control; 25 μm section at inter- aural 5.12 mm, bregma 1.32 mm; brown, DAB; blue,cresyl violet. Scale bar, 130 mm. Inset, 65 μm.(H and I) Elevated MFGE8 (H) in 5-month-old P301S-tau brains. Lysatesfrom cortex(Ctx), brain stem (BS), and cerebellum(Cb) of 5-month-old C57, 5-month-old P301S-tau, and 5-month-old Alz17 mice probed with anti-MFGE8; MFGE8 KO brain lysate isnegative control. rec, recombinant mouse MFGE8. (I) Densitometry of MFGE8 expression normalized to beta -actin. Significantlyhigher MFGE8 expression in P301S versus C57BU6 Ctx (**p < 0.01), BS (****p < 0.0001), Cb (**p < 0.01), andP301SversusALz17BS (****p < 0.0001). Mean ± SD, n = 3 independent preparations, two-way ANOVA, Bonferronicorrected.(J) Elevated MFGE8 expression in brain extracts from cortex (Ctx) of FTDP-17T patients with two differentMAPT mutations (P301L, +3) or sporadic Pick’s disease but not in extracts from patients with C9orf72hexanucleotide expansions and TDP-43 aggregates. No expression is found in the cerebellum, where tau pathology is absent in allcases. Human milk MFGE8 is a positive control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30134156), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MFG-E8 by Western Blot

Detection of Mouse MFG-E8 by Western Blot

MFGE8 regulates TG hydrolase activity through CES1D(A and B) (A) Representative western blot showing CES1D protein level in WT and Mfge8 KO primary enterocytes from three independent experiments. GAPDH was used as loading control. N = 8 mice per group. (B) Densitometric analysis of the western blots (including A).(C) Representative western blot showing CES1D protein level in WT and beta 5 KO primary enterocytes from two independent experiments. HSP90 was used as loading control. N = 5 WT and N = 4 beta 5 KO mice in total.(D) Densitometric analysis of the western blots of CES1D protein (including C).(E) TG hydrolase activity in WT, Ces1d KO, and Mfge8 KO primary enterocytes 1 h after incubation with rMFGE8 or RGE. N = 5 independent experiments.(F) Western blot of CES1D and MFGE8 protein levels in proximal small intestinal enterocytes of Mfge8 KO mice with transgenic inducible expression of MFGE8 in enterocytes (MFGE8 re-expressed, Vil rtTA+ TetO Mfge8+) and single transgenic (control), WT, and Mfge8 KO enterocyte controls. GAPDH was used as loading control.(G) Densitometric analysis of the blot presented in (F).(H) TG hydrolase activity in primary enterocytes isolated from the same groups of mice in (E) and (F). N = 3 mice in each group.(I and J) 3H signal in (I) the proximal jejunum and (J) serum 2 h after oral administration of [3H]oleic acid to WT, Mfge8 KO, Ces1d KO, and Mfge8/Ces1d double-KO mice. N = 3–4 mice in each group. All data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Data in (B), (D), and (G) were analyzed by unpaired t test. Data in (E) and (H) to (J) were analyzed by one-way ANOVA followed by Bonferroni’s post test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36924494), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MFG-E8 by Western Blot

Detection of Mouse MFG-E8 by Western Blot

MFGE8 regulates TG hydrolase activity through CES1D(A and B) (A) Representative western blot showing CES1D protein level in WT and Mfge8 KO primary enterocytes from three independent experiments. GAPDH was used as loading control. N = 8 mice per group. (B) Densitometric analysis of the western blots (including A).(C) Representative western blot showing CES1D protein level in WT and beta 5 KO primary enterocytes from two independent experiments. HSP90 was used as loading control. N = 5 WT and N = 4 beta 5 KO mice in total.(D) Densitometric analysis of the western blots of CES1D protein (including C).(E) TG hydrolase activity in WT, Ces1d KO, and Mfge8 KO primary enterocytes 1 h after incubation with rMFGE8 or RGE. N = 5 independent experiments.(F) Western blot of CES1D and MFGE8 protein levels in proximal small intestinal enterocytes of Mfge8 KO mice with transgenic inducible expression of MFGE8 in enterocytes (MFGE8 re-expressed, Vil rtTA+ TetO Mfge8+) and single transgenic (control), WT, and Mfge8 KO enterocyte controls. GAPDH was used as loading control.(G) Densitometric analysis of the blot presented in (F).(H) TG hydrolase activity in primary enterocytes isolated from the same groups of mice in (E) and (F). N = 3 mice in each group.(I and J) 3H signal in (I) the proximal jejunum and (J) serum 2 h after oral administration of [3H]oleic acid to WT, Mfge8 KO, Ces1d KO, and Mfge8/Ces1d double-KO mice. N = 3–4 mice in each group. All data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Data in (B), (D), and (G) were analyzed by unpaired t test. Data in (E) and (H) to (J) were analyzed by one-way ANOVA followed by Bonferroni’s post test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36924494), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MFG-E8 by Western Blot

Detection of Mouse MFG-E8 by Western Blot

Characterization of the surface functionalized mExo with FA. (A) Schematic illustration showing a process of surface engineering and Dox loading. The box with a blue dashed line demonstrates the modified chemical structure of 16:0 PE-FA obtained from Avanti Polar Lipids. (B) Size distribution of mExo species (mExo, mExo-FA, Dox@mExo, and Dox@mExo-FA). (C) TEM images of mExo, mExo-FA, Dox@mExo, and Dox@mExo-FA. Scale bar: 100 nm. (D) Western blotting analysis of TSG101, CD9, MFG-E8, calnexin, and casein. TSG101 and CD9, representative positive exosome markers; MFG-E8, milk-exosomal proteins; calnexin, negative marker of exosome; casein, representative contaminants from milk Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38031117), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse MFG‑E8 Antibody

Application
Recommended Usage

Simple Western

50 µg/mL
Sample: Recombinant Mouse (rm) MFG-E8

Western Blot

0.25 µg/mL
Sample: Mouse mammary gland tissue

Reviewed Applications

Read 3 reviews rated 5 using AF2805 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MFG-E8

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C-terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).

References

  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Stubbs, J.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8417.
  3. Hoffhines, A.J. et al. (2008) J. Biol. Chem. 284:3096.
  4. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  5. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  6. Hanayama, R. et al. (2002) Nature 417:182.
  7. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  8. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  9. Hanayama, R. et al. (2004) Science 304:1147.
  10. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  11. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  12. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  13. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  14. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.

Long Name

Milk Fat Globule EGF Factor 8

Alternate Names

BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, MFGE8, SED1

Entrez Gene IDs

4240 (Human); 17304 (Mouse)

Gene Symbol

MFGE8

UniProt

P21956

Additional MFG-E8 Products

Product Documents for Mouse MFG‑E8 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse MFG‑E8 Antibody

For research use only

Citations for Mouse MFG‑E8 Antibody

Customer Reviews for Mouse MFG‑E8 Antibody (3)

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  • Mouse MFG-E8 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Colon tissue
    Species: Mouse
    Verified Customer | Posted 10/01/2024
    Mouse MFG‑E8 Antibody AF2805
  • Mouse MFG-E8 Antibody
    Name: Juerg Messer
    Application: Immunohistochemistry
    Sample Tested: Alzheimer's disease brain
    Species: Mouse
    Verified Customer | Posted 06/30/2023
    Immunohistochemistry/fluorescence (Frozen sections). 10 µm cryostat sections from fresh frozen brain sample. Post-fixation with 4% formaldehyde for 15min. Blocking: PBS + 1% BSA +1% ovalbumin +10% normal donkey serum +0.3% LDAO +0.3M Glycine. Incubation buffer: PBS + 1% BSA +1% ovalbumin +10% normal donkey serum. Primary AB: goat anti-mouse MFG-E8 (#AF2805) 2 µg/ml, 2h at room temperature. DRAQ5 5µM (nuclei marker) together with primary AB. Secondary AB: Donkey anti-goat/AlexaFluor488 4 µg/ml, 1h at RT. Autofluorescence suppression: 4mM CuSO4 in 50mM ammoniumacetate buffer, pH 5, 30min at RT. Imaging: SlideView VS200 slide scanner with X-Cite NOVEM illumination.
    Mouse MFG‑E8 Antibody AF2805
  • Mouse MFG-E8 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Sperm
    Species: Porcine
    Verified Customer | Posted 09/07/2017
    Dilution: 1:1000 Secondary used: Goat IgG Horseradish Peroxidase-conjugated Antibody from R&D Systems.
    Mouse MFG‑E8 Antibody AF2805

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