Introduction
R&D Systems' Mouse Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY014) is a screening tool designed to simultaneously detect the relative phosphorylation of 39 different RTKs. The Kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. RTKs are captured by antibodies spotted on a nitrocellulose membrane. Levels of phospho-RTK are then assessed using an HRP-conjugated pan phospho-tyrosine antibody followed by chemiluminescence detection.
- Product Insert
- Troubleshooting Guide
- Assays for analytes represented in to the Mouse Phospho-Receptor Tyrosine Kinase Array Kit
- Western blotting antibodies for analytes related to the Mouse Phospho-Receptor Tyrosine Kinase Array Kit
| Simultaneously detect the relative phosphorylation of these RTKs in a single sample | ||
| EGF R | PDGF R alpha | VEGF R3 |
| ErbB2 | PDGF R beta | MuSK |
| ErbB3 | SCF R | EphA1 |
| ErbB4 | Flt-3 | EphA2 |
| FGF R2 (IIIc) | M-CSF R | EphA3 |
| FGF R3 | c-Ret | EphA6 |
| FGF R4 | Tie-1 | EphA7 |
| Insulin R | Tie-2 | EphA8 |
| IGF-I R | TrkA | EphB1 |
| Axl | TrkB | EphB2 |
| DtK | TrkC | EphB4 |
| Mer | VEGF R1 | EphB6 |
| HGF R | VEGF R2 | |
| MSP R | ||
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Lysis Buffer
- Array Buffers
- Wash Buffer
- Anti-Phospho-Tyrosine-HRP Detection Antibody
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.