14-3-3 beta/alpha Antibody - Azide Free

Novus Biologicals | Catalog # NB100-665

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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Mouse (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunohistochemistry-Paraffin (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all 14-3-3 beta/alpha Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P35213

Reactivity Notes

It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Localization

Cytoplasmic

Specificity

Specific for endogenous levels of the ~29 kDa 14-3-3 protein. Immunolabeling is blocked by preadsorption of the antibody with the peptide immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 beta/alpha Antibody - Azide Free

Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]

Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]

Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]

Applications for 14-3-3 beta/alpha Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~29 kDa.

Reviewed Applications

Read 1 review rated 4 using NB100-665 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 14-3-3 beta/alpha

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions. 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.

Alternate Names

14-3-3 alpha, 14-3-3 beta, 14-3-3 protein beta/alpha, brain protein 14-3-3, beta isoform, GW128, HS1, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, protein kinase C inhibitor protein-1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide, YWHAA

Entrez Gene IDs

7529 (Human); 54401 (Mouse); 56011 (Rat)

Gene Symbol

YWHAB

UniProt

P35213 (Rat)

Additional 14-3-3 beta/alpha Products

Product Documents for 14-3-3 beta/alpha Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for 14-3-3 beta/alpha Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for 14-3-3 beta/alpha Antibody - Azide Free (1)

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  • Name: Astrid Spannhoff
    Application: Western Blot
    Sample Tested: Mouse brain extract, Sample Amount: 10ul
    Species: Mouse
    Verified Customer | Posted 11/19/2010
    14-3-3 beta/alpha Antibody - Azide Free NB100-665

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