8-oxo-dG ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-62143

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, saliva, urine, plasma, seminal fluids, cell lysates, culture supernatants, DNA extracts (50 uL)

Sensitivity

0.59 ng/ml

Assay Range

0.94 - 60 ng/ml
View all 8-oxo-dG ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Specificity

The DNA Damage ELISA also detects 8-hydroxyguanosine (product of oxidative RNA damage) and 8-hydroxyguanine (product of oxidative DNA damage by hydroxyl radicals).

Precision

Intra-Assay Precision (Precision within an assay) The Intra-Assay Coefficient of variation of the DNA Damage ELISA has been determined to be 10%.

Inter-Assay Precision (Precision between assays) The Inter-Assay Coefficient of variation of the DNA Damage ELISA has been determined to be 10%

Scientific Data Images for 8-oxo-dG ELISA Kit (Colorimetric)

ELISA: 8-OHdG ELISA Kit (Colorimetric) [NBP2-62143] - Standard Reference Curve

Kit Contents for 8-oxo-dG ELISA Kit (Colorimetric)

  • Antibody
  • Antibody diluent
  • Conjugate
  • Conjugate diluent
  • Microtiter plate
  • Sample diluent
  • Standard
  • Stop solution 2
  • TMB Substrate
  • Wash buffer concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 8-oxo-dG

The production of 8-Hydroxyguanine (8-oxo-dG) is almost exclusively elicited by oxidative stress. Polymerases preferentially insert adenine opposite 8-oxo-dG. Therefore, oxidatively damaged adducts, without repair, are susceptible to G to T transitions.

Long Name

8-Hydroxyguanine

Alternate Names

8-OHG, 8oxodG

Additional 8-oxo-dG Products

Product Documents for 8-oxo-dG ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 8-oxo-dG ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Base Excision Repair

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