alcohol dehydrogenase Antibody (1X3C0)
Novus Biologicals | Catalog # NBP3-15822
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1X3C0 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Alcohol Dehydrogenase (P07327). MSTAGKVIKCKAAVLWELKKPFSIEEVEVAPPKAHEVRIKMVAVGICGTDDHVVSGTMVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLAIPQCGK
Reactivity Notes
Rat reactivity reported from a verified customer review.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for alcohol dehydrogenase Antibody (1X3C0)
Western Blot: alcohol dehydrogenase Antibody (1X3C0) [NBP3-15822]
Western Blot: alcohol dehydrogenase Antibody (1X3C0) [NBP3-15822] - Western blot analysis of extracts of Mouse lung, using alcohol dehydrogenase Rabbit mAb (NBP3-15822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western Blot: Rabbit alcohol dehydrogenase mAb (1X3C0) [NBP3-15822]
Western Blot: Rabbit alcohol dehydrogenase mAb (1X3C0) [NBP3-15822] - ADH Western blot of liver homogenates isolated from control and alcohol-fed rats. Image from a verified customer review.Applications for alcohol dehydrogenase Antibody (1X3C0)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Reviewed Applications
Read 1 review rated 5 using NBP3-15822 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: alcohol dehydrogenase
Alternate Names
ADH, alpha subunit, ADH1alcohol dehydrogenase 1A, alcohol dehydrogenase 1 (class I), alpha polypeptide, alcohol dehydrogenase 1A (class I), alpha polypeptide, Alcohol dehydrogenase subunit alpha, aldehyde reductase, EC 1.1.1, EC 1.1.1.1
Gene Symbol
ADH1A
Additional alcohol dehydrogenase Products
Product Documents for alcohol dehydrogenase Antibody (1X3C0)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for alcohol dehydrogenase Antibody (1X3C0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for alcohol dehydrogenase Antibody (1X3C0) (1)
5 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: Rat hepatocytesSpecies: RatVerified Customer | Posted 11/09/2024ADH W-B of liver homogenates isolated from control and alcohol-fed ratsBio-Techne ResponseThis review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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