Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25356

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Canine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2003 expressed in HEK293

Format

Azide and BSA Free
View all Alkaline Phosphatase, Tissue Non-Specific Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of Alkaline Phosphatase, Tissue Non-Specific.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images

Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] -

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] -

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] -

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] -

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) [NBP3-25356] -

Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) [NBP3-25356] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) [NBP3-25356] -

Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Tissue Non-Specific

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Gene Symbol

ALPL

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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