Key Product Details

Species Reactivity

Multi-Species

Applications

Flow Cytometry, CyTOF-ready

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 936809
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Product Specifications

Immunogen

Allophycocyanin isolated from red algae
Accession # P25054

Specificity

Detects Allophycocyanin in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Allophycocyanin Antibody (936809) [Alexa Fluor® 532]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Allophycocyanin

The APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cy5.5 and APC/Cy7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with tested mouse and human cells. The APC003 antibody can be used for separation of APC-antibody labeled cells or as a secondary step to amplify APC conjugated primary antibody signals (i.e. biotinylated APC003 as secondary step, followed by Streptavidin-APC).

Alternate Names

APC

Additional Allophycocyanin Products

Product Documents for Allophycocyanin Antibody (936809) [Alexa Fluor® 532]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Allophycocyanin Antibody (936809) [Alexa Fluor® 532]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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