Allophycocyanin Antibody

Catalog # Availability Size / Price Qty
MAB8927
MAB8927-SP
Detection of Allophycocyanin (APC) in Human PBMCs stained with APC-Conjugated Anti-Human CD3e by Flow Cytometry.
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Allophycocyanin Antibody Summary

Specificity
Detects Allophycocyanin in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 936809
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Allophycocyanin isolated from red algae
Accession # P25054
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Allophycocyanin (APC) in Human PBMCs stained with APC-Conjugated Anti-Human CD3e by Flow Cytometry. View Larger

Detection of Allophycocyanin (APC) in Human PBMCs stained with APC-Conjugated Anti-Human CD3e by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) stained with APC-conjugated Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # FAB100A) were stained with Mouse Anti-Allophycocyanin Monoclonal Antibody (Catalog # MAB8927, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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