Key Product Details

Species Reactivity

Multi-Species

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 936809
View all Allophycocyanin Primary Antibodies »

Product Specifications

Immunogen

Allophycocyanin isolated from red algae
Accession # P25054

Specificity

Detects Allophycocyanin in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Allophycocyanin Antibody

Detection of Allophycocyanin (APC) antibody in Human PBMCs stained with APC-Conjugated Anti-Human CD3e antibody by Flow Cytometry.

Detection of Allophycocyanin (APC) in Human PBMCs stained with APC-Conjugated Anti-Human CD3e by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMCs) stained with APC-conjugated Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # FAB100A) were stained with Mouse Anti-Allophycocyanin Monoclonal Antibody (Catalog # MAB8927, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Allophycocyanin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cell (PBMCs) stained with APC-conjugated Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # FAB100A)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Allophycocyanin

The APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cy5.5 and APC/Cy7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with tested mouse and human cells. The APC003 antibody can be used for separation of APC-antibody labeled cells or as a secondary step to amplify APC conjugated primary antibody signals (i.e. biotinylated APC003 as secondary step, followed by Streptavidin-APC).

Alternate Names

APC

UniProt

P25054

Additional Allophycocyanin Products

Product Documents for Allophycocyanin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Allophycocyanin Antibody

For research use only

Customer Reviews for Allophycocyanin Antibody

There are currently no reviews for this product. Be the first to review Allophycocyanin Antibody and earn rewards!

Have you used Allophycocyanin Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies