Alpha Amylase Antibody (HL1681)
Novus Biologicals | Catalog # NBP3-48800
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1681
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Product Specifications
Immunogen
Recombinant antibody of human Alpha Amylase.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Alpha Amylase Antibody (HL1681)
NBP3-48800-Alpha Amylase Antibody (HL1681)-
Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Amylase antibody [HL1681] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.NBP3-48800-Alpha Amylase Antibody (HL1681)-
Human pancreas (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Amylase antibody [HL1681] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.NBP3-48800-Alpha Amylase Antibody (HL1681)-
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Amylase antibody [HL1681] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.NBP3-48800-Alpha Amylase Antibody (HL1681)-
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Amylase antibody [HL1681] diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.NBP3-48800-Alpha Amylase Antibody (HL1681)-
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Amylase antibody [HL1681] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.NBP3-48800-Alpha Amylase Antibody (HL1681)-
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Amylase antibody [HL1681] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Western Blot: Alpha Amylase Antibody (HL1681) [NBP3-48800] -
Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alpha Amylase antibody [HL1681] (NBP3-48800) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Alpha Amylase Antibody (HL1681) [NBP3-48800] -
Human pancreas (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Amylase antibody [HL1681] (NBP3-48800) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Alpha Amylase Antibody (HL1681) [NBP3-48800] -
Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alpha Amylase antibody [HL1681] (NBP3-48800) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Alpha Amylase Antibody (HL1681) [NBP3-48800] -
Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Alpha Amylase antibody [HL1681] (NBP3-48800) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Alpha Amylase Antibody (HL1681) [NBP3-48800] -
Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alpha Amylase antibody [HL1681] (NBP3-48800) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Alpha Amylase Antibody (HL1681) [NBP3-48800] -
Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alpha Amylase antibody [HL1681] (NBP3-48800) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Alpha Amylase Antibody (HL1681)
Application
Recommended Usage
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alpha Amylase
Alternate Names
AMY2;AMY2A;AMY2B;AMY3;HXA;PA
Additional Alpha Amylase Products
Product Documents for Alpha Amylase Antibody (HL1681)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Alpha Amylase Antibody (HL1681)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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