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Scientific Data Images for ARF6 Antibody - BSA Free
Immunoprecipitation:ARF6 AntibodyNBP3-29164] -
Immunoprecipitation:ARF6 AntibodyNBP3-29164] -Detection of human ARF6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARF6 antibody NBP3-29164 (lot NBP3-29164-1) used for IP at 6 µg per reaction. ARF6 was also immunoprecipitated by rabbit anti-ARF6.. For blotting immunoprecipitated ARF6, NBP3-29164 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for ARF6 Antibody - BSA Free
Immunoprecipitation
Western Blot
Formulation, Preparation, and Storage
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Background: ARF6
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Additional ARF6 Products
Product Documents for ARF6 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for ARF6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ARF6 Antibody - BSA Free
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Q: I saw another protein purified product ARF6, which is expressed in E.coli. Did you expressed that protein with n-myristoyltransferase co-transformation E.coli?
A: We do not express the ARF6 protein with n-myristoyltransferase co-transformation E.coli.
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Q: We would like to buy a myristoylated Arf6 purified protein. If you have myristoylated Arf6 purified protein, please let me know.
A: Unfortunately, due to our expression system as well as Abnova's, we do not believe that these proteins have post-translational modifications similar to the endogenous protein
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Q: I saw another protein purified product ARF6, which is expressed in E.coli. Did you expressed that protein with n-myristoyltransferase co-transformation E.coli?
A: We do not express the ARF6 protein with n-myristoyltransferase co-transformation E.coli.
-
Q: We would like to buy a myristoylated Arf6 purified protein. If you have myristoylated Arf6 purified protein, please let me know.
A: Unfortunately, due to our expression system as well as Abnova's, we do not believe that these proteins have post-translational modifications similar to the endogenous protein