ARID1A Antibody - BSA Free

Novus Biologicals | Catalog # NB100-55334

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Mouse, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Western Blot, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (Negative)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ARID1A Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human AT rich interactive domain 1A (BRG1-associated factor 250kD) using the numbering given in entry NP_006006.3 (GeneID 8289).

Reactivity Notes

Mouse, Zebrafish reactivity reported from verified customer reviews.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARID1A Antibody - BSA Free

Western Blot: ARID1A Antibody [NB100-55334]

Western Blot: ARID1A Antibody [NB100-55334]

Western Blot: ARID1A Antibody [NB100-55334] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.1 ug/ml.
ARID1A-Antibody-Chromatin-Immunoprecipitation-NB100-55334-img0006.jpg
Western Blot: ARID1A Antibody [NB100-55334]

Western Blot: ARID1A Antibody [NB100-55334]

Western Blot: ARID1A Antibody [NB100-55334] - Mouse embryonic fibroblasts nuclear extract at 1:1000 dilution. (Nuclear extract instead of whole cell lysate provides much better signal.) Image provided via verified customer review.
Western Blot: ARID1A Antibody [NB100-55334]

Western Blot: ARID1A Antibody [NB100-55334]

Western Blot: ARID1A Antibody [NB100-55334] - Analysis of ARID1A in zebrafish embryos using anti-ARID1A antibody. Image from verified customer review.

Applications for ARID1A Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2000-1:10000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30337622). Immunoprecipitation Not recommended. ARID1A antibody validated for WB from verified customer reviews.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-55334 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARID1A

This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250A, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1

Entrez Gene IDs

8289 (Human)

Gene Symbol

ARID1A

UniProt

O14497 (Human)

Additional ARID1A Products

Product Documents for ARID1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ARID1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARID1A Antibody - BSA Free

Customer Reviews for ARID1A Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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50%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: zebrafish embryos
    Species: Other
    Verified Customer | Posted 03/23/2015
    ARID1A antibody in zebrafish
    ARID1A Antibody - BSA Free NB100-55334
  • Name: Xiaofeng Wang
    Application: Western Blot
    Sample Tested: mouse embryonic fibroblasts nuclear extract
    Species: Mouse
    Verified Customer | Posted 02/28/2014
    ARID1A Blotting in Mouse Embryonic Fibroblasts (MEFs)
    ARID1A Antibody - BSA Free NB100-55334

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