ASAH3 Overexpression Lysate

Name: ASAH3 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-07748
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBL1-07748

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for ASAH3 Overexpression Lysate

ASAH3 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC211332

Accession#: NM_133492

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.

Theoretical MW

30.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for ASAH3 Overexpression Lysate

Western Blot: ASAH3 Overexpression Lysate [NBL1-07748]

Western Blot: ASAH3 Overexpression Lysate (Adult Normal) [NBL1-07748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASAH3.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: ASAH3

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has ahighly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but notD-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels ofbioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids

Alternate Names

Acylsphingosine deacylase 3, Alkaline CDase 1, alkaline ceramidase 1, AlkCDase 1, ALKCDase1, ASAH3, EC 3.5.1.23, MGC138327, MGC138329, N-acylsphingosine amidohydrolase (alkaline ceramidase) 3, N-acylsphingosine amidohydrolase 3

Gene Symbol

ACER1

Additional ASAH3 Products

Product Documents for ASAH3 Overexpression Lysate

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Certificate of Analysis

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Product Specific Notices for ASAH3 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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