Beta 2 Adaptin Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29552

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Beta 2 Adaptin Primary Antibodies »

Product Specifications

Immunogen

Between 575 and 625

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Beta 2 Adaptin Antibody - BSA Free

Beta 2 Adaptin Antibody

Immunoprecipitation:Beta 2 Adaptin AntibodyNBP3-29552] -

Immunoprecipitation:Beta 2 Adaptin AntibodyNBP3-29552] -Detection of human Beta-2-adaptin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Beta-2-adaptin antibody NBP3-29552 (lot NBP3-29552-1) used for IP at 6 µg per reaction. Beta-2-adaptin was also immunoprecipitated by rabbit anti-Beta-2-adaptin.. For blotting immunoprecipitated Beta-2-adaptin, NBP3-29552 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Beta 2 Adaptin Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Beta 2 Adaptin

Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha-adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.

Alternate Names

Adapter-related protein complex 2 beta subunit, Adaptor protein complex AP-2 subunit beta, adaptor-related protein complex 2, beta 1 subunit, ADTB2adaptin, beta 2 (beta), AP105B, AP2-BETA, beta-2-adaptin, beta-adaptin, CLAPB1AP-2 complex subunit beta, Clathrin assembly protein complex 2 beta large chain, clathrin-associated/assembly/adaptor protein, large, beta 1, DKFZp781K0743, Plasma membrane adaptor HA2/AP2 adaptin beta subunit

Gene Symbol

AP2B1

Additional Beta 2 Adaptin Products

Product Documents for Beta 2 Adaptin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Beta 2 Adaptin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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