BSA Removal Antibody Purification Kit

Novus Biologicals | Catalog # 820-0100

Novus Biologicals
100% Guarantee

Key Product Details

Applications

Western Blot

Kit Type

Antibody Purification Kit

Product Summary for BSA Removal Antibody Purification Kit

Bovine Serum Albumin (BSA) is often added to purified antibodies as it is an effective stabilizer. However, when labeling antibodies, the BSA becomes a hindrance, as it directly competes with the antibody to attach to the label, greatly reducing the conjugation efficiency. Therefore, prior to undertaking labeling techniques, it is essential to remove the BSA.

Common commercial BSA removal techniques can involve many laborious steps. The AbSelect BSA Removal Kit is a simple 1 step, 10 minute method which effectively separates the BSA from the antibody and can be used on any antibody sub-type or species.

Benefits:
  • Easy-to-use
  • Only takes 10 minutes!
  • Centrifuge is the only equipment required
  • Also use to concentrate and exchange your buffer

Product Specifications

Application Notes

The AbSelectTM BSA Removal Kit can separate BSA from antibody solutions with antibody concentrations from 0.03mg/ml to 10mg/ml. Separation is more efficient at higher antibody concentrations. BSA can be effectively separated when present at concentrations of up to 0.5%. If BSA is present at higher concentrations, dilute the antibody mix with de-ionised, distilled water until BSA concentration is 0.5% or less.

Scientific Data Images for BSA Removal Antibody Purification Kit

Western Blot: BSA Removal Antibody Purification Kit [820-0100]

Kit Contents for BSA Removal Antibody Purification Kit

  • 1 vial AbSelect BSA Removal buffer
  • 1 vial AbSelect Resuspension buffer

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C.

Background: BSA Removal

Bovine Serum Albumin (BSA) is often added to purified antibodies as it is an effective stabilizer. However, when labeling antibodies, the BSA becomes a hindrance, as it directly competes with the antibody to attach to the label, greatly reducing the conjugation efficiency. Therefore, prior to undertaking labeling techniques, it is essential to remove the BSA.

Additional BSA Removal Products

Product Documents for BSA Removal Antibody Purification Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BSA Removal Antibody Purification Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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