Biological ActivityCommonly used laboratory reagent
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Cytosolic Ca2+ sensitive indicator, Fura-2 was loaded in RBL-2H3 cells and excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm. ER Ca2+ was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry via STIM1/Orai1 pathway into the cells upon addition of 2 mM extracellular CaCl2.