Capicua Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30691

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Capicua Primary Antibodies »

Product Specifications

Immunogen

between 1548 and 1608

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Capicua Antibody - BSA Free

Capicua Antibody

Immunoprecipitation: Rabbit Capicua pAb [NBP3-30691]

Immunoprecipitation: Rabbit Capicua pAb [NBP3-30691] - Samples: Whole cell lysate (1 mg per IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CIC/Capicua antibody (NBP3-30691) used for IP at 6 µg per reaction. CIC/Capicua was also immunoprecipitated by a second antibody against a different epitope of CIC/Capicua (NB110-59905). For blotting immunoprecipitated CIC/Capicua, A301-204A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Capicua Antibody

Western Blot: Rabbit Capicua pAb [NBP3-30691]

Western Blot: Rabbit Capicua pAb [NBP3-30691] - Samples: Whole cell lysate (50 µg) from MCF-7, Jurkat, HEK293T, RKO, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CIC/Capicua antibody (NBP3-30691) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for Capicua Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Capicua

CIC, a human homolog of Drosophila capicua, encodes a high mobility group box transcription factor and is fused to a double homeodomain gene DUX4 as a result of a recurrent chromosomal translocation. It is involved in epidermal growth factor receptor (EGFR) signaling, developmental patterning, and cell fate. In humans, CIC may be involved in granule cell lineage, cerebellar development, and medulloblastoma tumorigenesis. Recent evidence indicates that CIC is involved in Spinocerebellar Ataxia Type 1 (SCA-1) where it interacts directly with the product of the SCA-1 gene Ataxin-1.

Alternate Names

capicua homolog (Drosophila), KIAA0306capicua (Drosophila) homolog, protein capicua homolog

Gene Symbol

CIC

Additional Capicua Products

Product Documents for Capicua Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Capicua Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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