Recombinant Mouse CCL28 Protein

Novus Biologicals | Catalog # NBP2-35169

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

Q9JIL2

Applications

Bioactivity, SDS-PAGE
View all CCL28 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 111 amino acids corresponding to CCL28 Source: E. coli

Uniprot ID: Q9JIL2

Amino Acid Sequence: SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL28 as determined by LAL method.

Predicted Molecular Mass

12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL28 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration range of 1.0-10.0 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CCL28 Protein

SDS-PAGE: Recombinant Mouse CCL28 Protein [NBP2-35169]

SDS-PAGE: Recombinant Mouse CCL28 Protein [NBP2-35169]

SDS-Page: Mouse CCL28 Protein [NBP2-35169]

Formulation, Preparation, and Storage

NBP2-35169
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL28

CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 mRNA is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83% identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils.

Alternate Names

CCL28, MEC, VIC, MEC/CCL28

Gene Symbol

CCL28

UniProt

Q9JIL2

Additional CCL28 Products

Product Documents for Recombinant Mouse CCL28 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CCL28 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Chemokines and Receptors

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