CD277/BTN3A1 Antibody (BT3.1) - BSA Free

Novus Biologicals | Catalog # NBP2-00227

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BT3.1

Format

BSA Free
View all CD277/BTN3A1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is CD277 (B7 family, Butyrophilin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD277/BTN3A1 Antibody (BT3.1) - BSA Free

Flow Cytometry: CD277/BTN3A1 Antibody (BT3.1) - BSA Free [NBP2-00227]

Flow Cytometry: CD277/BTN3A1 Antibody (BT3.1) - BSA Free [NBP2-00227]

Flow Cytometry: CD277/BTN3A1 Antibody (BT3.1) [NBP2-00227] - Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD277 Purified (filled histogram). Cells in the lymphocyte gate were used for analysis.

Applications for CD277/BTN3A1 Antibody (BT3.1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:10-1:500
Application Notes
This BT3.1 (20.1, BT3.1) antibody has been tested by flow cytometric analysis of normal human peripheral blood mononuclear cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This T3.1 (20.1, BT3.1) antibody has been reported for use in immunoprecipitation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD277/BTN3A1

The BT3.1 monoclonal antibody recognizes BT3.1, BT3.2 and BT3.3. The BT family of proteins is a subgroup of the Ig superfamily (IgSF). BT is a glycoprotein that forms a major component of the milk fat globule molecule. In addition to BT, there are six other genes that have been discovered which have been sub-divided into the BT2 (BT2.1, BT2.2, and BT2.3) and BT3 (BT3.1, BT3.2, and BT3.3) families. BT3.1, BT3.2, and BT3.3 share 95% mRNA identity. BT3 molecules are constitutively expressed on the cell surface of antigen-presenting cells, and the IgV-like domain of BT3.1 is sufficient for interaction with its counter-receptor. BT3.1 is expressed by T cells, B cells and CD14+ cells.

Alternate Names

BT3.1, BTF5, BTN3A1, CD277

Gene Symbol

BTN3A1

Additional CD277/BTN3A1 Products

Product Documents for CD277/BTN3A1 Antibody (BT3.1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD277/BTN3A1 Antibody (BT3.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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