Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 4H8E5

Format

Azide and BSA Free
View all CD5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen is a peptide made to the amino acid region CD5

Specificity

CD5/Lyt-1, Mr 67 kDa

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for CD5 Antibody (4H8E5) - Azide and BSA Free

Flow Cytometry: CD5 Antibody (4H8E5) - Azide and BSA Free [NBP1-26703]

Flow Cytometry: CD5 Antibody (4H8E5) - Azide and BSA Free [NBP1-26703]

Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the PE conjugate of NBP1-26703. Double staining of 0.2 ug/1^6 cells from BALB/c spleen with rat anti-mouse CD5-R-PE and rat anti-mouse CD19-FITC Clone 6D5), following which small lymphocytes were gated and analyzed using a flow cytometer.
Flow Cytometry: CD5 Antibody (4H8E5) - Azide and BSA Free [NBP1-26703]

Flow Cytometry: CD5 Antibody (4H8E5) - Azide and BSA Free [NBP1-26703]

Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the Allophycocyanin conjugate of NBP1-26703. Multiple staining of C57BL/6 splenocytes.
Flow Cytometry: CD5 Antibody (4H8E5) - Azide and BSA Free [NBP1-26703]

Flow Cytometry: CD5 Antibody (4H8E5) - Azide and BSA Free [NBP1-26703]

Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the Biotin conjugate of NBP1-26703. Multiple staining of C57BL/6 splenocytes.
Flow Cytometry: CD5 Antibody (4H8E5) - Azide and BSA Free [NBP1-26703]

Flow Cytometry: CD5 Antibody (4H8E5) - Azide and BSA Free [NBP1-26703]

Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the FITC conjugate of NBP1-26703. Multiple staining of C57BL/6 splenocytes.

Applications for CD5 Antibody (4H8E5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/10 cells

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD5

CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (1-3) It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (13, 14)

Alternate Names

CD5

Gene Symbol

CD5

Additional CD5 Products

Product Documents for CD5 Antibody (4H8E5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD5 Antibody (4H8E5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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