CD84/SLAMF5 Antibody (CD84.1.21) [FITC]

Novus Biologicals | Catalog # NB100-64062

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # CD84.1.21
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Product Specifications

Immunogen

Mouse pre-B cell line 300.19 transfected with CD84 cDNA

Localization

Type I membrane protein

Specificity

NB100-65929 recognizes the human CD84 cell surface antigen, a highly glycosylated cell surface antigen of 64-82kD. CD84 is predominantly expressed by B lymphocytes, monocytes and macrophages, although lower levels of expression have also been reported on T cells, granulocytes and platelets.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD84/SLAMF5 Antibody (CD84.1.21) [FITC]

CD84/SLAMF5 Antibody (CD84.1.21) [FITC] Flow Cytometry: CD84/SLAMF5 Antibody (CD84.1.21) [FITC] - [NB100-64062] -

Flow Cytometry: CD84/SLAMF5 Antibody (CD84.1.21) [FITC] - [NB100-64062] -

Flow Cytometry: CD84/SLAMF5 Antibody (CD84.1.21) [FITC] - [NB100-64062] - Figure A. Alexa Fluor 700 conjugated mouse anti human CD14 and RPE conjugated mouse IgG1 isotype control. Figure B. Alexa Fluor 700 conjugated mouse anti human CD14 and RPE conjugated mouse anti human CD84. All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock. Data acquired on the ZE5 Cell Analyzer.

Applications for CD84/SLAMF5 Antibody (CD84.1.21) [FITC]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1% Bovine Serum Albumin

Preservative

0.09% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD84/SLAMF5

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production. CD84 is expressed on mature B cells and B cell lines but not on plasma cell lines. Immunohistochemical studies demonstrate that it strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T cells. Cellular expression does not significantly increase after activation.

Long Name

SLAM Family Member 5

Alternate Names

CD84, Ly-9B, SLAMF5

Entrez Gene IDs

8832 (Human)

Gene Symbol

CD84

UniProt

Additional CD84/SLAMF5 Products

Product Documents for CD84/SLAMF5 Antibody (CD84.1.21) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD84/SLAMF5 Antibody (CD84.1.21) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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