Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # 30-H12

Format

Azide and BSA Free
Loading...

Product Specifications

Immunogen

Mouse thymus or spleen

Specificity

Mouse CD90.2/Thy-1.2 alloantigen, Mr 18 kDa

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for CD90.2 Antibody (30-H12) - Azide and BSA Free

Flow Cytometry: CD90.2 Antibody (30-H12) - Azide and BSA Free [NBP1-28036]

Flow Cytometry: CD90.2 Antibody (30-H12) - Azide and BSA Free [NBP1-28036]

Flow Cytometry: CD90.2 Antibody (30-H12) [NBP1-28036] - Analysis using the Allophycocyanin conjugate of NBP1-28036. Multiple staining of BALB/c splenocytes.

Applications for CD90.2 Antibody (30-H12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

<= 1 ug/10^6 cells

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD90.2

CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily, consisting of a single V-set domain. (1-3) The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL). (2-5)

Alternate Names

CD90, CD90 antigen, CDw90, FLJ33325, Thy-1 antigen, Thy-1 cell surface antigen, thy-1 membrane glycoprotein, Thy-1 T-cell antigen

Gene Symbol

THY1

Additional CD90.2 Products

Product Documents for CD90.2 Antibody (30-H12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD90.2 Antibody (30-H12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD90.2 Antibody (30-H12) - Azide and BSA Free

Customer Reviews for CD90.2 Antibody (30-H12) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review CD90.2 Antibody (30-H12) - Azide and BSA Free and earn rewards!

Have you used CD90.2 Antibody (30-H12) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for CD90.2 Antibody (30-H12) - Azide and BSA Free

Showing  1 - 1 of 1 FAQ Showing All
  • Q: I am looking for an anti-mouse Thy1.2 to use in immunofluorescence. However, my samples are formalin-fixed paraffin embedded (FFPE). Have any of your antibodies been tested for reactivity against fixed epitopes that would be worth trying on my samples?

    A: We have not tested these antibodies in IHC-P yet, however, I do not see any reason why these products should not work in this application! Because this product worked well in IHC-Fr and FLOW applications wherein the antibodies bind the antigen in relatively native state/sub-cellular form, we strongly believe that it should work in IHC-P also! However, you will certainly need to play around the antigen retrieval timings (depending upon for how long your tissues were kept for fixation in formalin) to get your staining procedure optimized.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies
Loading...