Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, In vitro assay

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # 30-H12
View all CD90.2 Primary Antibodies »

Product Specifications

Immunogen

Mouse thymus or spleen

Specificity

Mouse CD90.2/Thy-1.2 alloantigen, Mr 18 kDa

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for CD90.2 Antibody (30-H12) [FITC]

Flow Cytometry: CD90.2 Antibody (30-H12) [FITC] [NBP1-28037] - 0.3 ug/10^6 BALB/c splenocytes were double-stained with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19-R-PE. Small lymphocytes were then gated and analyzed on a flow cytometer
Flow Cytometry: CD90.2 Antibody (30-H12) [FITC] [NBP1-28037] - 1ug on Balb/c Splenocytes

Applications for CD90.2 Antibody (30-H12) [FITC]

Application
Recommended Usage

Flow Cytometry

1 ug/10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Reviewed Applications

Read 1 review rated 5 using NBP1-28037 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD90.2

CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily, consisting of a single V-set domain. (1-3) The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL). (2-5)

Alternate Names

CD90, CD90 antigen, CDw90, FLJ33325, Thy-1 antigen, Thy-1 cell surface antigen, thy-1 membrane glycoprotein, Thy-1 T-cell antigen

Gene Symbol

THY1

Additional CD90.2 Products

Product Documents for CD90.2 Antibody (30-H12) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD90.2 Antibody (30-H12) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD90.2 Antibody (30-H12) [FITC] (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used CD90.2 Antibody (30-H12) [FITC]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • CD90.2 Antibody (30-H12) [FITC]
    Name: James Xiao
    Application: Flow Cytometry
    Sample Tested: Mouse spleen cells
    Species: Mouse spleen and IL1-R KO Mouse
    Verified Customer | Posted 01/03/2018
    Cells were pre-gated with live/dead and FSC-A/W to exclude the dead cells and cell doublets.
    The single cell suspension was isolated from a spleen of an IL1R-KO (knockout) mouse and stained with FITC-Conjugated CD90.2 Antibody for 30 minutes. Cells were analyzed with flow cytometer.
    CD90.2 Antibody (30-H12) [FITC] NBP1-28037

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies