Rat CEBP Delta ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-70027

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat C/EBP(delta) in samples. No significant cross-reactivity or interference between Rat C/EBPIdelta) and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.58%

Inter-Assay Precision (Precision between assays) CV% 4.92%

Recovery for Rat CEBP Delta ELISA Kit (Colorimetric)

Recovery

90-105%

Linearity

Rat CEBP Delta ELISA Kit (Colorimetric)

Scientific Data Images for Rat CEBP Delta ELISA Kit (Colorimetric)

Rat CEBP Delta ELISA Kit (Colorimetric)

ELISA: Rat CEBP Delta ELISA Kit (Colorimetric) [NBP2-70027] -

ELISA: Rat CEBP Delta ELISA Kit (Colorimetric) [NBP2-70027] - Standard Curve Reference

Kit Contents for Rat CEBP Delta ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CEBP Delta

C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBP delta may increase binding activity, whereas phosphorylation of the alpha and beta family members may decrease binding affinity. C/EBP delta is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Alternate Names

C/EBP-delta, CCAAT/enhancer binding protein (C/EBP), delta, CCAAT/enhancer-binding protein delta, CELF, CRP3, NF-IL6-betaC/EBP delta, Nuclear factor NF-IL6-beta

Gene Symbol

CEBPD

Additional CEBP Delta Products

Product Documents for Rat CEBP Delta ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat CEBP Delta ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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