CENPE Antibody (CA8) - BSA Free

Novus Biologicals | Catalog # NBP3-43561

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Cynomolgus Monkey

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CA8

Format

BSA Free
View all CENPE Primary Antibodies »

Product Specifications

Immunogen

The original mouse parental antibody 118G03 was generated by immunizing SJL mice with recombinant human CENPE/TNFRSF17-Fc chimera (R&D 193-Fc) by using the RIMMS method (Rapid immunization multiple sites). A chimeric version of this antibody was generated and it is called CA8. (Uniprot# Q02224)

Specificity

This antibody binds human and cynomolgus monkey CENPE and does not bind TACI or BAFF-R proteins. CENPE acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. CENPE expression is highest on terminally differentiated B cells. CENPE is involved in mediating the survival of plasma cells for maintaining long-term humoral immunity. It also activates NF-kappa-B and JNK. The expression of CENPE has been linked to a number of cancers, autoimmune disorders, and infectious diseases.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CENPE Antibody (CA8) - BSA Free

CENPE Antibody (CA8) - BSA Free CENPE Antibody (CA8) - BSA Free

Immunocytochemistry/ Immunofluorescence: CENPE Antibody (CA8) - BSA Free [NBP3-43561] -

Immunocytochemistry/ Immunofluorescence: CENPE Antibody (CA8) - BSA Free [NBP3-43561] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on coverslips stained with the chimeric version of this antibody, NBP3-43562 (1:100 dilution), for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43562, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
CENPE Antibody (CA8) - BSA Free CENPE Antibody (CA8) - BSA Free

Flow Cytometry: CENPE Antibody (CA8) - BSA Free [NBP3-43561] -

Flow Cytometry: CENPE Antibody (CA8) - BSA Free [NBP3-43561] - Daudi cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the chimeric version of this antibody, NBP3-43562 (blue line), at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.

Applications for CENPE Antibody (CA8) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CENPE

Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq]

Long Name

Centromere-associated protein E

Alternate Names

CENP-E, Kinesin-7

Gene Symbol

CENPE

Additional CENPE Products

Product Documents for CENPE Antibody (CA8) - BSA Free

Certificate of Analysis

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Product Specific Notices for CENPE Antibody (CA8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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