Citron Kinase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-28740

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Citron Kinase Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1925 and 1975 of human citron (rho-interacting, serine/threonine kinase 21) using the numbering given in entry NP_009105.1 (GeneID 11113).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Citron Kinase Antibody - BSA Free

Immunoprecipitation: Citron Kinase Antibody [NBP1-28740]

Immunoprecipitation: Citron Kinase Antibody [NBP1-28740]

Immunoprecipitation: Citron Kinase Antibody [NBP1-28740] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Citron antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Citron was also immunoprecipitated by rabbit anti-Citron antibody BL5459, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for Citron Kinase Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Citron Kinase

Citron is a dual specificity serine/threonine protein kinase and a putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It is required for KIF14 localization to the central spindle and midbody and may play a role in cytokinesis. Citron also plays an important role in the regulation of cytokinesis and the development of the central nervous system. [Uniprot]

Alternate Names

CITK, citron (rho-interacting, serine/threonine kinase 21), Citron Rho-Interacting Kinase, Citron Rho-Interacting Serine/Threonine Kinase, CRIK, KIAA0949, MCPH17, Rho-interacting Serine/Threonine kinase 21, serine/threonine kinase 21, Serine/threonine-protein kinase 21, STK21

Entrez Gene IDs

11113 (Human)

Gene Symbol

CIT

UniProt

O14578 (Human)

Additional Citron Kinase Products

Product Documents for Citron Kinase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Citron Kinase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Citron Kinase Antibody - BSA Free

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