Recombinant Human CLC Protein

Novus Biologicals | Catalog # NBP2-61319

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Western Blot, Bioactivity
View all CLC Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 198 amino acids corresponding to CLC Source: E. coli

Uniprot ID: Q9UBD9

Amino Acid Sequence: LNRTGDPGPG PSIQKTYDLT RYLEHQLRSL AGTYLNYLGP PFNEPDFNPP RLGAETLPRA TVDLEVWRSL NDKLRLTQNY EAYSHLLCYL RGLNRQAATA ELRRSLAHFC TSLQGLLGSI AGVMAALGYP LPQPLPGTEP TWTPGPAHSD FLQKMDDFWL LKELQTWLWR SAKDFNRLKK KMQPPAAAVT LHLGAHGF

Purity

>98%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CLC as determined by LAL method.

Predicted Molecular Mass

22.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CLC protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of > 2.5 x 10^5 IU/mg in the presence of human CNTF R alpha.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CLC Protein

SDS-PAGE: Recombinant Human CLC Protein [NBP2-61319]

SDS-PAGE: Recombinant Human CLC Protein [NBP2-61319]

SDS-Page: Recombinant Human NR6/CLC Protein [NBP2-61319]

Formulation, Preparation, and Storage

NBP2-61319
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLC

The CLCF1 gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1(CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor

Long Name

Cardiotrophin-like Cytokine

Alternate Names

BSF-3, CISS2, CLCF1, NNT-1, NR6

Gene Symbol

CLCF1

Additional CLC Products

Product Documents for Recombinant Human CLC Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human CLC Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
IL-6 Family

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