CometAssay Alkaline Control Cells

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.

A: The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.

A: The control cells, Catalog # 4256-010-CC, need to be run through the entire protocol for Catalog # 4250-050-K in order to see comets.

A: The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.

A: CometAssay Control Cells were prepared using an immortalized human lymphocyte line.

A: A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).

A: The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.

A: Failure to lyse, denature in alkali, or properly perform electrophoresis may generate poor results.   To improve results:

1. Verify lysis of cells. Lysis Solution should be stored long term at room temperature and chilled to 4 °C prior to use. Lysis Solution stored long term at 4 °C will precipitate. Try overnight lysis at 4 °C.
2. Perform alkali denaturation for 20 minutes at room temperature or for at least 1 hour at 4 °C. Prepare Alkaline Solution fresh from sodium hydroxide pellets.
3. Electrophoresis conditions are also critical for optimal performance. Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). For optimal results, use a CometAssay Electrophoresis system (Catalog # 4250-050-ES). For insufficient electrophoresis time, increase time up to 1 hour at 4 °C for alkaline electrophoresis.

A: The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks. Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.

A: A pellet may not be visible after centrifugation due to the low number of cells.

A: The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.