COPG Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99186

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all COPG Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human COPG.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for COPG Antibody - Azide and BSA Free

Western Blot: COPG Antibody [NBP2-99186]

Western Blot: COPG Antibody [NBP2-99186]

Western Blot: COPG Antibody [NBP2-99186] - Anti-COPG1 rabbit polyclonal antibody at 1:500 dilution. Lane A: THP1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 105 kDa. Observed band size: 105 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: COPG Antibody [NBP2-99186]

Immunoprecipitation: COPG Antibody [NBP2-99186]

Immunoprecipitation: COPG Antibody [NBP2-99186] - COPG1 was immunoprecipitated using: Lane A: 0.5 mg THP-1 Whole Cell Lysate Lane B: 0.5 mg HUT-78 Whole Cell Lysate Lane C: 0.5 mg U87MG Whole Cell Lysate 1 ul anti-COPG1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-COPG1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 96 kDa. Observed band size: 96 kDa

Applications for COPG Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COPG

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors

Alternate Names

coat protein gamma-cop, coatomer protein complex, subunit gamma, coatomer protein complex, subunit gamma 1, COPG1coatomer subunit gamma, FLJ21068, Gamma-coat protein, Gamma-COP

Gene Symbol

COPG1

Additional COPG Products

Product Documents for COPG Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for COPG Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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