DBF4 Antibody (0S8I5)

Novus Biologicals | Catalog # NBP3-15903

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0S8I5 expressed in HEK293
View all DBF4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DBF4 (Q9UBU7). MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DBF4 Antibody (0S8I5)

Western Blot: DBF4 Antibody (0S8I5) [NBP3-15903]

Western Blot: DBF4 Antibody (0S8I5) [NBP3-15903]

Western Blot: DBF4 Antibody (0S8I5) [NBP3-15903] - Western blot analysis of extracts of various cell lines, using DBF4 Rabbit mAb (NBP3-15903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for DBF4 Antibody (0S8I5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DBF4

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle

Alternate Names

ASKchif, DBF4 homolog (S. cerevisiae), DBF4ACHIF, DBF4-type zinc finger-containing protein 1, ZDBF1DBF-type containing 1

Gene Symbol

DBF4

Additional DBF4 Products

Product Documents for DBF4 Antibody (0S8I5)

Certificate of Analysis

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Product Specific Notices for DBF4 Antibody (0S8I5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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