Q: Customer requests if there is any information if catalog # 20830002 has been used in WB yet? Is there any recommended protocol for the use in WB for this antibody? Additionally, the customer would like to know how NBL1-08067 has been detected in WB? With an antibody to DEPP or with an antibody to the tag?

A: With regards to your first question, catalog # 20830002 has not been tested and validated for WB and we do not recommend our customers to use our antibodies in an application in which they haven't been tested before. To answer your second question, Anti-DDK antibody is used to detect NBL1-08067.

DEPP1 Overexpression Lysate

Novus Biologicals | Catalog # NBL1-08067

Submit a review

Product Documents

100% Guarantee

Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for DEPP1 Overexpression Lysate

DEPP1 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC202009

Accession#: NM_007021

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

23.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for DEPP1 Overexpression Lysate

Western Blot: DEPP1 Overexpression Lysate [NBL1-08067]

Western Blot: C10orf10 Overexpression Lysate (Adult Normal) [NBL1-08067] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C10orf10.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: DEPP1

The expression of this gene is induced by fasting as well as by progesterone. The protein encoded by this gene contains a t-synaptosome-associated protein receptor (SNARE) coiled-coil homology domain and a peroxisomal targeting signal. Production of the encoded protein leads to phosphorylation and activation of the transcription factor ELK1. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

Alternate Names

C10orf10, chromosome 10 open reading frame 10, Decidual protein induced by progesterone, DEPP, fasting induced, Fasting-induced gene protein, fasting-induced protein, FIG, protein DEPP

Gene Symbol

C10ORF10

Additional DEPP1 Products

Product Documents for DEPP1 Overexpression Lysate

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DEPP1 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customer Reviews for DEPP1 Overexpression Lysate

There are currently no reviews for this product. Be the first to review DEPP1 Overexpression Lysate and earn rewards!

Have you used DEPP1 Overexpression Lysate?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image