DNA Polymerase delta, catalytic subunit Antibody (4B6I3)

Novus Biologicals | Catalog # NBP3-16358

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4B6I3 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1000-1107 of human DNA Polymerase delta, catalytic subunit (P28340). GGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA Polymerase delta, catalytic subunit Antibody (4B6I3)

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (4B6I3) [NBP3-16358]

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (4B6I3) [NBP3-16358]

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (4B6I3) [NBP3-16358] - Western blot analysis of extracts of various cell lines, using DNA Polymerase delta, catalytic subunit Rabbit mAb (NBP3-16358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for DNA Polymerase delta, catalytic subunit Antibody (4B6I3)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNA Polymerase delta, catalytic subunit

DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex

Alternate Names

CDC2, CDC2 homolog, DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, DNA-directed polymerase delta 1, EC 2.7.7.7, POLD, polymerase (DNA directed), delta 1, catalytic subunit (125kD), polymerase (DNA directed), delta 1, catalytic subunit 125kDa

Gene Symbol

POLD1

Additional DNA Polymerase delta, catalytic subunit Products

Product Documents for DNA Polymerase delta, catalytic subunit Antibody (4B6I3)

Certificate of Analysis

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Product Specific Notices for DNA Polymerase delta, catalytic subunit Antibody (4B6I3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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