DNAJC8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87015

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DNAJC8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDGRVDSWRNFQANTKGKKEKKNRTFLRPPKVK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAJC8 Antibody - BSA Free

Immunohistochemistry-Paraffin: DNAJC8 Antibody [NBP1-87015]

Immunohistochemistry-Paraffin: DNAJC8 Antibody [NBP1-87015]

Immunohistochemistry-Paraffin: DNAJC8 Antibody [NBP1-87015] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Simple Western: DNAJC8 Antibody [NBP1-87015]

Simple Western: DNAJC8 Antibody [NBP1-87015]

Simple Western: DNAJC8 Antibody [NBP1-87015] - Simple Western lane view shows a specific band for DNAJC8 in 0.2 mg/ml of Ntera-2 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: DNAJC8 Antibody [NBP1-87015]

Simple Western: DNAJC8 Antibody [NBP1-87015]

Simple Western: DNAJC8 Antibody [NBP1-87015] - Electropherogram image of the corresponding Simple Western lane view. DNAJC8 antibody was used at 1:5 dilution on Ntera-2 lysate(s) respectively.
DNAJC8 Antibody - BSA Free Western Blot: DNAJC8 Antibody - BSA Free [NBP1-87015]

Western Blot: DNAJC8 Antibody - BSA Free [NBP1-87015]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line NTERA-2

Applications for DNAJC8 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Ntera-2 lysate(s), separated by Size, antibody dilution of 1:5, apparent MW was 41 kDa.

Reviewed Applications

Read 1 review rated 4 using NBP1-87015 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNAJC8

DjC8 (DnaJ subfamily C member 8) belongs to the Hsp40 (heat shock protein 40) family of proteins that function as co-chaperones to Hsp70.Hsp40 proteins are classified into 3 main subfamilies (A, B, and C). The families are defined by the presence of particular domains which include a highly conserved alpha-helical N-terminal domain termed the J-domain, a glycine/phenylalanine-rich region, a cysteine-rich region, and a C-terminal beta-sheet containing domain. Djc8 is part of subfamily C that bears only the J-domain. DjC8 is also known as splicing protein spf31, and DNAJC8.

Alternate Names

DnaJ (Hsp40) homolog, subfamily C, member 8, dnaJ homolog subfamily C member 8, HSPC331, SPF31Splicing protein spf31

Gene Symbol

DNAJC8

Additional DNAJC8 Products

Product Documents for DNAJC8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DNAJC8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DNAJC8 Antibody - BSA Free (1)

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  • DNAJC8 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Tissue Lysates
    Species: Human
    Verified Customer | Posted 04/11/2019
    DNAJC8 Antibody - BSA Free NBP1-87015

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