Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DNMT1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human DNA-Methyltranserase 1 using the numbering given in entry NP_001370.1 (GeneID 1786).

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit DNMT1 Antibody - BSA Free (NB100-392) is a polyclonal antibody validated for use in WB and IP. Anti-DNMT1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DNMT1 Antibody - BSA Free

Western Blot: DNMT1 Antibody [NB100-392]

Western Blot: DNMT1 Antibody [NB100-392]

Western Blot: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: DNMT1 Antibody [NB100-392]

Western Blot: DNMT1 Antibody [NB100-392]

Western Blot: DNMT1 Antibody [NB100-392] - Dnmt1 in mouse brain cells. Image submitted by a verified customer review.
Immunoprecipitation: DNMT1 Antibody [NB100-392]

Immunoprecipitation: DNMT1 Antibody [NB100-392]

Immunoprecipitation: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 in HeLa nuclear extracts [50 ug/lane (A), 10 ug input, or 1 mg NB 100-392 IP (B)] using NB 100-392. DNMT1 immunoprecipitated with NB 100-392 and detected with NB 100-392 using 0.2 ug/ml. 2 second ECL exposure.
Immunoprecipitation: DNMT1 Antibody [NB100-392]

Immunoprecipitation: DNMT1 Antibody [NB100-392]

Immunoprecipitation: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for IP at 3 ug per reaction. For blotting immunoprecipitated DNMT1, NB100-392 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for DNMT1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 4 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
DNMT1 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 3 using NB100-392 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNMT1

Inactivation of tumor suppressor genes is a key component of the development of all common forms of human cancers. Aberrant methylation may contribute to many of the changes, both physiological and pathological, associated with aging, including tumor development. Hypermethylation of CpG island-containing promoters is associated with the inactivation of genes important to tumor angiogenesis and progression. DNA methytransferase (DNMT1) is necessary for the maintenance of the methylation process.

Long Name

DNA [Cytosine-5-]-Methyltransferase 1

Alternate Names

CXXC9, MCMT

Entrez Gene IDs

1786 (Human)

Gene Symbol

DNMT1

UniProt

NP_001370.1 (Human)

Additional DNMT1 Products

Product Documents for DNMT1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DNMT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DNMT1 Antibody - BSA Free

Customer Reviews for DNMT1 Antibody - BSA Free (1)

3 out of 5
1 Customer Rating
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3 Stars
100%
2 Stars
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1 Stars
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Lysate from homogenized mouse brain cells
    Species: Mouse
    Verified Customer | Posted 07/17/2014
    Western blot for Dnmt1 in mouse brain cells
    DNMT1 Antibody - BSA Free NB100-392

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